Objective To investigate the effect and mechanism of ginsenoside Rg3 on the proliferation and metastasis of cervical cancer cells in vitro. Methods Cervical cancer cells HeLa were incubated with different concentrations (0.00, 0.12, 0.24, 0.48 mmol/L) of ginsenoside Rg3 for 24 h. The survival rate of HeLa cells was detected by CCK-8 method. Transwell assay was used to detect the migration and invasion of HeLa cells. Western blot was used to detect E-cadherin, N-cadherin, vimentin, Toll like receptor (TLR) 4, myeloid differentiation factor (MyD) 88, phosphorylated nuclear transcription factor p-NF-κB p65 protein expression. Results After treatment with ginsenoside Rg3 (0.12, 0.24, 0.48 mmol/L), the survival rate, migration number, invasion number and protein expression of N-cadherin, vimentin, TLR4, MyD88, p-NF-κB p65 in HeLa cells were significantly decreased (P< 0.05), and expression of E-cadherin protein was significantly increased (P<0.05) in a concentration-dependent manner. Conclusion Ginsenoside Rg3 can inhibit the proliferation and metastasis of cervical cancer cells in vitro, and the mechanism may be related to the inhibition of NF-κB signaling pathway.