Turmeric spent, a byproduct of turmeric processing industries, was used as a source to prepare nanofibers (NF).The chem. treatments methods followed by acid hydrolysis accompanied with high pressure homogenization were used to prepare NF.The resulting turmeric nanofibers (TNF) were characterized by Fourier transform IR spectroscopy (FT-IR), Raman spectroscopy, SEM (SEM), transmission electron microscopy (TEM), at. force microscopy (AFM), X-ray diffraction (XRD) and thermogravimetric anal. (TGA/DTA).The TNF presented needle like structure, high thermal stability, an average width of 38.5 nm, average length of 245.7 nm, and giving an aspect ratio (L/D) of 23.15.The prepared TNF showed pronounced antimicrobial activity against Bacillus cereus, Escherichia Coli, Salmonella typhimurium and Staphylococcus aureus and also registered good antioxidant activity.The results showed that TNF were successfully obtained from turmeric spent and might be potentially applied in different fields, such as pharmaceutical, biol. active species, nutraceuticals, components for food industries and bionanocomposites.