Jiamusi

Research has indicated that aberrant amygdala connectivity is a crucial neurobiological underpinning of juvenile depression. This study aimed to further identify resting-state dynamic functional connectivity (dFC) changes between the lateral amygdala (LA), medial amygdala (MeA), and whole-brain voxels, as well as their correlations with clinical symptoms in adolescents with depression.

The bilateral LA and MeA were selected as regions of interest, and the sliding window method was applied to investigate dFC changes in 47 adolescents with depression, compared with 35 sex-, age-, and education-matched healthy controls (HCs). Subsequently, the relationship between the dFC variability values and Patient Health Questionnaire-9 and Simplified Coping Styles Questionnaire (SCSQ) scores in the patient group was investigated.

Compared with HCs, there was reduced dFC variability between both the left LA and left MeA and right precentral gyrus; both the right LA and right MeA and left median cingulate and paracingulate gyri; and the right MeA and right cuneus in adolescents with depression. Furthermore, the dFC variability between the right MeA and right cuneus correlated positively with negative coping scores of the SCSQ (r = 0.414, p = 0.005) in adolescents with depression.

These findings confirm abnormal dynamic functional pathways of amygdala subregions in adolescent depression, and suggest that abnormal dFC may be involved in the neural mechanisms underlying the pathogenesis of adolescent depression.

Human cytomegalovirus (HCMV), a prevalent double-stranded DNA enveloped virus, poses a threat to immunocompromised individuals. The current clinical detection methods are insufficient in sensitivity, highlighting the need for more effective approaches.

We designed and screened RPA primers and crRNA based on the UL123 gene of HCMV. Evaluate the HCMV-RPA-CRISPR detection method using cloned plasmids and the whole-genome samples of HCMV-infected cells. Conduct RPA-CRISPR/Cas12a reactions with 48 clinical samples and compare the results with those of PCR-Fluorescent Probe Method in clinical applications and the qPCR method for detecting the UL123 gene.

The optimized RPA-CRISPR system exhibited high sensitivity and specificity for HCMV detection. The positive rate of clinical sample detection was approximately 20.5 % (6/48) higher than that of the clinical detection method.

Currently, the sensitivity and early detection of HCMV in clinical settings are still limited. The UL123 gene of HCMV is characterized by high transcription in the early stage and high conservation. The RPA-CRISPR/Cas12a technology exhibits high sensitivity in detecting the HCMV UL123 gene, and it is expected to provide a more effective method for the early specific detection of HCMV infection.

Tin disulfide (SnS₂) with its high capacity and suitable interlayer spacing, is a promising anode for sodium-ion batteries (SIBs), but its substantial volume expansion during sodium insertion and sluggish ion and electron transfer kinetics in bulk materials limit its broader application. Herein, SnS₂/NiS₂ particles are encapsulated in hollow nitrogen-doped carbon nanofibers (SnS₂/NiS₂@HCNFs) by the combination of coaxial electrostatic spinning and carbonization/sulfurization processes. The hollow channel structure of the carbon skeleton creates a three-dimensional pathway that facilitates rapid electron transmission and provides buffer space for volume changes, enhancing structural stability. Additionally, the interface electric field generated by the SnS₂/NiS₂ heterojunction accelerates Na⁺ transfer, as supported by findings from density functional theory (DFT) calculations and galvanostatic intermittent titration techniques (GITT). Together, the hollow structure and heterojunction contribute to improved reaction kinetics. As a result, the SnS₂/NiS₂@HCNFs composite exhibits outstanding cycling stability with a capacity of 315 mA h g^-1 over 1000 cycles at 2 A g^-1. Moreover, the assembled SnS₂/NiS₂@HCNFs//Na₃V₂(PO₄)₃ full-cell delivers a high reversible capacity of 186 mA h g^-1 after 500 cycles at 1 A g^-1. This study offers a valuable approach for the rational design of heterostructured anodes aimed at enhancing the efficiency of SIBs.