Ufuk University

ADAMTSs are extracellular matrix metalloproteinases that mainly process extracellular matrix components and closely related tumorigenesis. ADAMTS-8 is an anti-angiogenic member of the family and is dysregulated in common cancers. The tumor suppressor function of the ADAMTS-8 has been demonstrated in colorectal cancer. Although ADAMTS-8 plays a critical role in tumor progression, transcriptional regulatory features haven't been studied yet.

The human ADAMTS-8 promoter was cloned into the pMetLuc Reporter vector. Basal promoter activity and the effect of the IL-6 on ADAMTS-8 promoter activity were determined by transient transfection assays in SW480 cells. QRT-PCR and Western blot analyses assessed the impact of IL-6 on ADAMTS-8 mRNA and protein expressions. Functional binding of the specific transcription factors to the ADAMTS-8 promoter region was evaluated by ChIP qPCR and EMSA.

Our results demonstrated that the ADAMTS-8 promoter includes multiple binding sites for transcription factors that could be activated in the inflammatory pathways. IL-6 stimulation increased ADAMTS-8 promoter activity, also mRNA, and protein expressions. Pathway inhibition studies showed that IL-6-mediated induction of ADAMTS-8 was achieved through p38/MAPK, NF-κB, PI3K, and SAPK/JNK pathways. STATs, Elk-1, and c-Jun functionally bind to the ADAMTS-8 promoter region.

It can be concluded that inflammation is a strong positive regulator of the ADAMTS-8 gene.

This study aimed to: (1) compare sleep quality and sleep-related parameters in a Turkish adult population with and without CDS and ADHD symptoms; and (2) re-examine the relationship between CDS and sleep by exploring potential associations between sleep problems and CDS symptoms, while also examining the separate roles of sluggishness and daydreaming, as well as possible gender differences.

The study included 274 participants (age:18-35, 70.4 % female). The Adult CDS Ratings Scale, the Adult ADHD Self-Report Scale (ASRS), and the Pittsburgh Sleep Quality Index were used. The data were analyzed using SPSS 22 and AMOS 23. Statistical analyses included ANOVA with post-hoc Tamhane's test, Mann-Whitney U test, Spearman Correlation Analysis, and structural equation modeling with path analysis. Linear regression analysis was conducted to identify factors influencing daydreaming.

The study yielded four key findings: CONCLUSIONS: This study is among the first to explore the relationship between CDS symptoms and sleep problems in young adults, accounting for ADHD. Our findings highlight the significant association of hyperactivity/impulsivity and daydreaming with sleep quality, as well as the relationship between poor sleep quality and increased daydreaming and sluggishness, though to a lesser extent than ADHD symptoms. Notably, gender differences emerged, with poor sleep quality affecting CDS symptoms primarily in women, while sluggishness decreased with age in men. These results underscore the importance of considering sleep issues in the context of CDS and ADHD, particularly with attention to gender-specific patterns.