Wenzhou University

Hyperglycemic microenvironment is a major cause of impaired wounds healing in diabetes. Real-time and rapid monitoring of the local glucose is essential for effective caring and intervening in diabetic wounds. Herein, an activatable fluorescence nanoprobe (Zn-CDs) was developed for rapid response to glucose. Zn-CDs were ligand complexes formed by Zn²⁺ and near-infrared emitting carbon dots (CDs). The fluorescence of CDs was quenched due to their aggregation induced by Zn²⁺, and gluconic acid, which was a strong chelating agent, restored the fluorescence signals and imaging the local glucose level of the wounds. Zn-CDs were synthesized in a simple process and showed high glucose selectivity and excellent sensitivity (detection limit as low as 23.11 μM). The favorable glucose monitoring performance of Zn-CDs was demonstrated in vitro, proving its stable imaging ability in the complex cellular microenvironments. The utility and reliability of Zn-CDs was further evaluated in diabetic wounds, demonstrating that the fluorescence nanoprobe was able to rapidly turn on the fluorescence imaging positively correlating with the local glucose in pathological wounds. Therefore, Zn-CDs provides a feasible strategy to monitoring wounds pathology in a cost-effective manner as a promising candidate for efficiently managing diabetic wounds.

In this paper, we study the dynamics of a delayed predator-prey model with harvesting and prey refuge. For the model without time delays, we show the stability of boundary and positive equilibria, given the existence of bionomic equilibrium, and provide the optimal harvesting policy. For the model with time delays, we show the local stability of the positive equilibrium for four different time delay cases, give the existence of Hopf bifurcation near the positive equilibrium, and prove the direction and stability of bifurcating periodic solutions. Ecologically, via numerical simulations, we find that the synergistic effects of time delay, refuge and harvesting can have complex effects on population dynamics. One of the most important results indicates that the increase of prey refuge or prey harvesting rate can eliminate the periodic solutions induced by time delay, while the increase in predator harvesting rate can maintain this periodic phenomenon.

We studied the viromes of three dominant mosquito species in Wenzhou, a coastal city in Zhejiang Province, using metavirome sequencing, with 18 viral families identified. Viral sequences were verified by RT-PCR. The JEV E gene was most closely related to the 1988 Korean strain. DENV sequences were most closely related to the 1997 Australian strain. CHIKV-E1-1 was most closely related to the 1983 Senegal strain and belonged to West African genotype CHIKV. Remarkably, this is the first time that a West African genotype of CHIKV has been detected in Zhejiang Province. Mutations in the CHIKV-E1-1 protein A226V may increase infectivity in Ae. albopictus. Three non-conservative mutations of CHIKV-E1-1 (D45H, D70H and V290D) may have an impact on the function. In conclusion, our study reveals the diversity of mosquito-borne viruses and potential emerging outbreaks in the southeast coastal region of China, providing new perspectives for mining the ecological characterization of other important arboviruses.