Henan Normal University

Bacillus velezensis (B. velezensis) HF-14,109 is an aerobic Gram-positive bacterium isolated from the gut of healthy carp (Cyprinus carpio L.). Here, we sequenced and annotated the genome of HF-14,109, identified and classified its enzyme-producing genes and secondary metabolite biosynthesis gene clusters (BGCs), and verified the inhibitory effects on pathogenic bacteria in vitro. Results suggested that HF-14,109 had a circular 4,214,172 bp genome that contains 4,392 predicted genes with an average length and GC content of 851.56 bp and 46.94%, respectively. A total of 210 non-coding RNAs, 8 CRISPR sequences, and 152 tandem repeats were predicted. Based on a CAZy database analysis, HF-14,109 contains 93 genes encoding enzymes for carbohydrate-related processes, 38 of which were glycoside hydrolase genes and divided into 20 families. Based on enzyme predictions, HF-14,109 had the capability to hydrolyze high molecular carbohydrates such as starch, disaccharides such as lactose and sucrose, and non-starch polysaccharides such as β-glucan, mannan, fructan and xylan. Twelve BGCs for producting secondary metabolites were identified by antiSMASH analysis, 5 of which were predicted to encode polyketide synthases (PKSs) and non-ribosomal peptide synthetases (NRPSs), indicating that HF-14,109 could produce multiple secondary metabolites. The genome of HF-14,109 contained numerous genes encoding glycoside hydrolases and BGCs for producting secondary metabolites. Besides, the HF-14,109 could inhibit pathogenic bacteria such as E. tarda, A. hydrophila, S. aurenus, and E. coli in vitro. In conclusion, our results demonstrate that HF-14,109 has the effects of hydrolyzing non-starch polysaccharides and inhibiting pathogenic bacteria, which lays a solid foundation for elucidating its antibacterial and enzyme-producing mechanisms, and is expected to be developed as a probiotic for aquaculture feed in the future.

Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) are key downstream effectors of the Hippo pathway that regulate organ size, tissue homeostasis, and cancer development. YAP/TAZ play crucial regulatory roles in organ growth, cell proliferation, cell renewal, and regeneration. Mechanistically, YAP/TAZ influence the occurrence and progression of liver regeneration (LR) through various signaling pathways, including Notch, Wnt/β-catenin, TGF-β/Smad. While the activation of YAP/TAZ can promote the regeneration of damaged liver tissue, their mechanisms of action may differ under various LR conditions. Furthermore, excessive activation of YAP/TAZ may also lead to severe liver damage, manifesting as alcoholic hepatitis, liver fibrosis, and even liver cancer. Here, we review the role and mechanisms of YAP/TAZ in LR and liver disease, highlighting the potential for advancements in clinical diagnosis and treatment targeting YAP/TAZ in these contexts.

The phytohormone salicylic acid (SA) serves as a crucial signaling molecule within the realm of plant immunity, playing an indispensable role in both local and systemic acquired resistance (SAR). N-hydroxypipecolic acid (NHP), a derivative of L-lysine, is integral to the induction of SAR. Recent investigations have illuminated the intricate manner in which NHP orchestrates the establishment of SAR in conjunction with the immune signal SA.

To further explore the mechanisms governing the synergistic regulation of SAR by SA and NHP, we conducted an extensive phosphoproteomic analysis aimed at identifying the phosphoproteins modulated either commonly or uniquely by SA and NHP, employing a phosphoproteomics platform built upon high-resolution mass spectrometry. Our study revealed a total of 133 phosphopeptides, derived from 115 distinct proteins, exhibiting exclusive responsiveness to NHP treatment. In contrast, 229 phosphopeptides sourced from 204 proteins demonstrated exclusive sensitivity to SA treatment. Additionally, the phosphorylation status of 215 proteins, including numerous kinases, phosphatases, transcription factors, and proteins implicated in membrane trafficking, was commonly modulated by both SA and NHP.

This investigation offers detailed insights into the key phosphoproteins influenced either collectively or specifically by SA and NHP, thereby enabling further exploration of the mechanisms underlying the synergistic regulation of immune responses orchestrated by these two potent molecules.