To establish a method for determining chlorhexidine in compounded spray powder intermediate. The chlorhexidine in compounded spray powder intermediate was determined by UV spectrophotometry with chlorhexidine hydrochloride as control at wavelength 258 nm. A good linear relationship was obtained in 0.006-0.01 mg/L (r = 0.9995, average recovery 99.76%, and RSD 0.17%). The method was feasible.