To establish a method for determining chlorhexidine in compounded spray powder intermediate.The chlorhexidine in compounded spray powder intermediate was determined by UV spectrophotometry with chlorhexidine hydrochloride as control at wavelength 258 nm.A good linear relationship was obtained in 0.006-0.01 mg/L (r = 0.9995, average recovery 99.76%, and RSD 0.17%).The method was feasible.