The Third Xiangya Hospital of Central South University

Viral myocarditis (VMC) is a common inflammatory disease of the myocardium that is characterized mainly by inflammatory cell infiltration and cardiomyocyte necrosis. Coxsackievirus B3 (CVB3) is a common cause of VMC, although major progress has been made in the treatment of VMC, the long-term prognosis is still not ideal and further research is needed. Non-coding RNAs (ncRNAs) are RNA molecules without coding functions and include microRNAs (miRNAs), long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs), which play extensive regulatory roles in gene expression; however, their mechanisms of action in CVB3-induced VMC remain incompletely understood. Here, we review the currently known roles of various ncRNAs in CVB3-induced VMC models, with a focus on cell death, inflammation and viral replication, with the aim of providing a reference for their therapeutic or vaccine development for the treatment of VMC.

A comprehensive chemical investigation of the EtOAc extract derived from the dried branches and leaves of Phyllanthus franchetianus H. Lév had successfully resulted to the isolation of six undescribed cleistanthane diterpenoids phyllanfranins A-F (1-6), along with three known compounds phyllarheophol C (7), phyacioid C (8), and spruceanol (9). The chemical structures of these compounds were elucidated by combined means of HRESIMS, 1D and 2D NMR spectra, together with ECD calculations. The absolute configuration of phyllanfranin A (1) was established by single crystal X-ray diffraction analysis. Notably, phyllanfranin F (6) represents the first ent-cleistanthane diterpenoid with the unique 6/6/6/6 tetracyclic system occurring in nature. Additionally, all the isolates were evaluated for anti-inflammatory activities. As a result, compounds 4 and 8 showed notable inhibitory activity against NO production in LPS-stimulated macrophages RAW264.7 cells, with IC₅₀ values of 19.03 and 18.14 μM, respectively.

Bone implant-associated bacterial infection is a common cause of transplant failure. Chemodynamic therapy (CDT) has significant antibacterial efficacy by producing reactive oxygen species (ROS). However, the ROS permeability is restricted by the natural barrier of bacterial biofilms. Herein, a hard-soft magnetic biphasic nanoparticle (CF@MF) was synthesized by in-situ growth of the soft MnFe₂O₄ shell on the hard CoFe₂O₄ core. Then, the CF@MF was incorporated into poly-l-lactide powders to prepare porous scaffolds by selective laser sintering. The scaffold exhibited superior magnetothermal conversion efficiency, which was attributed to the interfacial exchange coupling of CF@MF. Importantly, the scaffold effectively dispersed biofilms by magnetothermal therapy (MTT), favoring numerous ROS pre-generated by CDT to enter the interior to kill bacteria. This study provided a synergistic MTT/CDT strategy to eradicate deep bacterial infections in biomedical application.