Hubei University

N6-methyladenosine (m6A) is the most prevalent post-transcriptional modification in eukaryotic RNA and is also present in various viral RNAs, where it plays a crucial role in regulating the viral life cycle. However, the molecular mechanisms through which viruses regulate host RNA m6A methylation are not fully understood. In this study, we reveal that SARS-CoV-2 and HCoV-OC43 infection enhance host m6A modification by activating the mTORC1 signalling pathway. Specifically, the viral non-structural protein nsp14 upregulates the expression of S-adenosylmethionine synthase MAT2A in an mTORC1-dependent manner. This mTORC1-MAT2A axis subsequently stimulates the synthesis of S-adenosylmethionine (SAM). The increase of SAM then enhances the m6A methylation of host RNA and facilitates viral replication. Our findings uncover a molecular mechanism by which viruses regulate host m6A methylation and provide insights into how SARS-CoV-2 hijacks host cellular epitranscriptomic modifications to promote its replication.

The soybean-based (SBB) solvent, derived from renewable feedstocks, exhibits significant potential for CO₂ chemisorption due to its sustainability, non-toxicity, biodegradability, and low regeneration energy consumption.However, the practical application of unrefined SBB at high concentrations is limited by its high content of low-reactive components (e.g., acidic and low-solubility amino acids).To address this limitation, we developed an isoelec. precipitation-based formulation engineering strategy to enhance the CO₂ capture performance of this absorbent.By selectively removing amino acids through pH-controlled precipitation, the concentration of active amino acids in the optimized solvent (SBB-M) increased by over 60 %, enabling its use at a refined and relatively high concentration (∼3 M).And the absorption capacity of the SBB-M solution has significantly increased by 16.6 times compared with that of the SBB solvent.Kinetic anal. revealed a reaction order of 1.68-1.8 and average activation energy of 24.7 kJ·mol^-1 for the CO₂ absorption into SBB-M aqueous solution, indicating efficient chemisorption ability.Remarkably, the regeneration energy of 3 M SBB-M was only 1.98 GJ·ton^-1 CO₂, approx. 48 % of that required for 5 M monoethanolamine (MEA), underscoring its energy-saving superiority.This formulation engineering of SBB represents a breakthrough in developing sustainable amino acid solvents for cost-effective and scalable carbon capture.

The sequential deposition process has been widely employed to fabricate perovskite solar cells (PSCs) due to its favorable operability. However, the random orientation of perovskite (PVK) films resulting from the rapid and unregulated solid-liquid reaction poses a significant challenge to enhancing the performance of PSCs prepared via the sequential deposition process. This study proposes a strategy for manipulating the arrangement pattern of lead iodide (PbI₂) crystals to regulate the orientation and uniformity of PVK films. Polyvinylidene fluoride (PVDF) molecules are introduced into the PbI₂ solution to manipulate the arrangement of PbI₂ through their interactions. The enhanced PbI₂ crystals with the (301) plane create favorable conditions for the preferential growth of PVK crystals, significantly improving the crystallinity and uniformity of the PVK film. Ultimately, we achieved formamidinium-rich PSCs with photoelectric conversion efficiencies (PCEs) of 24.84 % for a millimeter-scale device (active area of 0.09 cm²) and 21.15 % for a centimeter-scale device (active area of 1 cm²). Furthermore, the reduction in defect state density and internal stress within the film resulted in unencapsulated devices exhibiting remarkable stability under various harsh environmental conditions.