To establish a RP-HPLC method for determination of angiotensin amide and its related substances, the Waters XBridge C18 column was used. A mobile phase A(20 mmol/L potassium dihydrogen phosphate buffer(adjusting pH value to 3.0 with orthophosphoric acid)) and mobile phase B(50% acetonitrile) were adopted. The ratio of mobile phase A and B was adjusted to keep the retention time of angiotensin amide and related compounds at about 10 min. The flow rate was 1 mL/min and the detection wavelength was 220 nm, and the volume of injection was 20μL. The calibration curve was linear in the range of 0.10∼0.90 mg/mL(r=0.999 9, n=5). The intra- and inter-day precision(RSD) was 0.39% and 0.66% resp. The average recovery was 98.5%, and the RSD was 0.26%(n=9). The method was completely validated with respect to specificity, accuracy, precision and stability. The results indicated that the method was suitable to the anal. of angiotensin amide and its related substances, and also could be used in the quality control of angiotensin amide and its preparations