Guangzhou Baiyunshan Qixing Pharmaceutical Co., Ltd.

Introduction. Positive effects have been observed when the traditional Chinese medicine Hua Tuo Zai Zao Wan (HTZZW) has been used for the treatment of atherosclerosis (AS), although with an unclear mechanism. Methods. ApoE-/- C57/BALB mice were used to determine the efficacy of HTZZW by blood lipid biochemical analysis and histopathology H&E staining. qPCR and western blot were used to determine the expression of METTL3/14 and NF-κB. Results. High-fat diet-fed ApoE-/- mice that consumed HTZZW exhibited significantly smaller plaque areas and significantly decreased unstable collagen areas in the aortic arch as well as significantly lower blood levels of total cholesterol, triglycerides, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol compared with the control group. Consumption of HTZZW significantly decreased the proportion of Mφ1 in the peripheral blood. HTZZW not only inhibited the expression of m6A methyltransferases METTL14, METTL3, and overall RNA methylation level, but it also decreased the m6A modification level on specific sites of NF-κB mRNA. Conclusion. HTZZW significantly alleviated the progression of AS by regulating the expression of the m6A methyltransferases METTL14 and METTL3 in macrophages, eliminating m6A modifications of NF-κB mRNA, influencing the stability of NF-κB mRNA, and ultimately resulting in the deactivation of inflammatory macrophages.

To investigate the pharmacological effect of Yifuzhixue Pill (YFZXP).

PPT, RT, PT, platelet counts and platelet adhesive rate were examined after administration of YFZXP. In other hand, the functions of isolated rabbit's uteri were also examined to determine whether they were responsible to the administration of YFZXP.

YFZXP could enhance the intrinsic blood coagulating function and stimulate the contraction of smooth muscles of uterus including increasing the rhythms and strength. The stimulation effect of YFZXP on smooth muscles of uterus exhibited no selections between the uteri treated with or without estradiol. It was speculated that the increased intrinsic blood coagulating and uterus' contraction induced by YFZXP were contributed to its anti-bleeding effect in uterus.

Objective To observe the microfiltration of different types of ceramic membrane for aqueous extract of Rhizoma Chuanxiong, and to select the optimal type of ceramic membranes.Methods The aqueous extract of Rhizoma Chuanxiong was microfiltered by inorganic ceramic membranes in the pore diameter of 50 nm, 200 nm, and 500 nm, resp.The characters, total solids, and active ingredients of the aqueous extract were analyzed and compared before and after microfiltration.Results The aqueous extract turned into clarified liquid from turbid liquid after microfiltration with the three types of the inorganic ceramic membrane.The removal rate for total solids was 25.7 %, 26.5 % and 25.9 %, and the loss rate for ferulic acid was 12%, 6 % and 4 %, resp. for the inorganic ceramic membranes in the pore diameter of 50 nm, 200 nm, and 500 nm.Conclusion Both the yield of dry solid material and ferulic acid are stable during the three middle - scale tests, indicating that the aperture ceramic membrane in the pore diameter of 200 nm is an optimal selection for the purification and refining of aqueous extract of Rhizoma Chuanxiong.