Hainan Bikai Pharmaceutical Co. Ltd.

Three new labdane-type diterpenoids, callicapene M3-M5 (1-3) were isolated from the Callicarpa macrophylla Vahl. Their structures were identified by spectroscopic method. The isolated compounds were evaluated for inhibitory activity on NO production in LPS-activated RAW 264.7 macrophage cells by using MTT assays. Compounds 1-3 showed potent inhibitory activity, with IC₅₀ value of 48.15, 46.31 and 38.72 μM respectively.

Four new sesquiterpenoids, 4,8-dioxo-6β-hydroxyl-7β,11-epoxycarabrane (1), 4,8-dioxo-6β-hydroxyl-7α,11-epoxycarabrane (2), wenyujinins Q and R (3-4), and nine known sesquiterpenoids (5-13) were isolated from the Curcuma wenyujin (C. wenyujin) dreg.Their structures and relative configurations were elucidated using 1D, 2D NMR, and HR-ESI-MS data.All the compounds were isolated for the first time from the C. wenyujin dreg and evaluated for their antibacterial and antifungal activities.Compounds 3, 5-8 exhibited strong broad-spectrum antifungal activities against tested nine pathogenic fungi.