The clinical trial was designed to determine the efficacy (sensitivity and specificity) of the IUrisure test compared to the gold standard cystoscopy and pathology in patients under monitoring for recurrence of urothelial carcinoma.

Start Date01 Jun 2023

Sponsor / Collaborator

Wuhan Ammunition Life-tech Co., Ltd.

NCT05668793 / Not yet recruitingNot Applicable

Methylation Detection of KCNA3 and OTOP2 Genes in Plasma for the Auxiliary Diagnosis of Hepatocellular Carcinoma: a Clinical Trial

The goal of this observational study is to test the clinical efficacy of "GNB4 and Riplet gene methylation combined detection kit (fluorescence PCR method)"in hepatocellular carcinoma auxiliary diagnosis. The main questions it aims to answer are:
How consistent are the test results of the kit with the clinical reference diagnostic criteria?
Sanger sequencing can visually show whether each sample contains methylation sites, so in this clinical trial, the kit results were compared with Sanger sequencing results to analyze the reagent's accuracy in detecting GNB4 and Riplet gene methylation.
Each participant is required to provide no less than 10 ml of blood to complete the kit test.

Start Date01 Jan 2023

Sponsor / Collaborator

Wuhan Ammunition Life-tech Co., Ltd.

[+1]

NCT05680077 / CompletedNot Applicable

Methylation Detection of KCNA3 and OTOP2 Genes in Plasma for the Auxiliary Diagnosis of Esophageal Cancer/Precancerous Lesions: a Clinical Trial

The goal of this observational study is to test the clinical efficacy of "KCNA3 and OTOP2 gene methylation combined detection kit (fluorescence PCR method)"in esophageal cancer and high-grade esophageal neoplasia auxiliary diagnosis.The main questions it aims to answer are:
How consistent are the test results of the kit with the clinical reference diagnostic criteria?
Sanger sequencing can visually show whether each sample contains methylation sites, so in this clinical trial, the kit results were compared with Sanger sequencing results to analyze the reagent's accuracy in detecting KCNA3 and OTOP2 gene methylation.
Each participant is required to provide no less than 10 ml of blood to complete the kit test.

Start Date28 Oct 2022

Sponsor / Collaborator

Wuhan Ammunition Life-tech Co., Ltd.

[+1]

100 Clinical Results associated with Wuhan Ammunition Life-tech Co., Ltd.

0 Patents (Medical) associated with Wuhan Ammunition Life-tech Co., Ltd.

Literatures (Medical) associated with Wuhan Ammunition Life-tech Co., Ltd.

31 Dec 2024·Epigenetics

Plasma methylated GNB4 and Riplet as a novel dual-marker panel for the detection of hepatocellular carcinoma

Article

Author: Huang, Yue ; Zhang, Lianglu ; Zhao, Yanteng ; Jin, Huifang ; Xie, Ying ; Wang, Shaochi ; Chen, Liyinghui ; Wan, Kangkang ; Zhao, Lei ; Yang, Qiankun ; Zhang, Wei ; Dong, Lanlan

Early detection of hepatocellular carcinoma (HCC) can greatly improve the survival rate of patients. We aimed to develop a novel marker panel based on cell-free DNA (cfDNA) methylation for the detection of HCC. The differentially methylated CpG sites (DMCs) specific for HCC blood diagnosis were selected from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases, then validated by the whole genome bisulphite sequencing (WGBS) of 12 paired HCC and paracancerous tissues. The clinical performance of the panel was evaluated using tissue samples [32 HCC, chronic liver disease (CLD), and healthy individuals] and plasma cohorts (173 HCC, 199 CLD, and 98 healthy individuals). The combination of G protein subunit beta 4 (GNB4) and Riplet had the optimal area under the curve (AUC) in seven candidates through TCGA, GEO, and WGBS analyses. In tissue validation, the GNB4 and Riplet showed an AUC of 100% with a sensitivity and specificity of 100% for detecting any-stage HCC. In plasma, it demonstrated a high sensitivity of 84.39% at 91.92% specificity, with an AUC of 92.51% for detecting any-stage HCC. The dual-marker panel had a higher sensitivity of 78.26% for stage I HCC than alpha-fetoprotein (AFP) of 47.83%, and a high sensitivity of 70.27% for detecting a single tumour (size ≤3 cm). In conclusion, we developed a novel dual-marker panel that demonstrates high accuracy in detecting HCC, surpassing the performance of AFP testing.

20 Jul 2024·Chinese Medical Journal

Genome-wide methylation profiling identified methylated KCNA3 and OTOP2 as promising diagnostic markers for esophageal squamous cell carcinoma

Article

Author: Lin, Han ; Wan, Kangkang ; Zhou, Jun ; Gao, Ye ; Tian, Bo ; Lu, Chaojing ; Zhou, Siwei ; Zhang, Xun ; Xin, Lei ; Li, Zhaoshen ; Wang, Luowei ; Bian, Yan ; Zhang, Yanhui ; Chen, Hezhong

AbstractBackground:Early detection of esophageal squamous cell carcinoma (ESCC) can considerably improve the prognosis of patients. Aberrant cell-free DNA (cfDNA) methylation signatures are a promising tool for detecting ESCC. However, available markers based on cell-free DNA methylation are still inadequate. This study aimed to identify ESCC-specific cfDNA methylation markers and evaluate the diagnostic performance in the early detection of ESCC.Methods:We performed whole-genome bisulfite sequencing (WGBS) for 24 ESCC tissues and their normal adjacent tissues. Based on the WGBS data, we identified 21,469,837 eligible CpG sites (CpGs). By integrating several methylation datasets, we identified several promising ESCC-specific cell-free DNA methylation markers. Finally, we developed a dual-marker panel based on methylated KCNA3 and OTOP2, and then, we evaluated its performance in our training and validation cohorts.Results:The ESCC diagnostic model constructed based on KCNA3 and OTOP2 had an AUC of 0.91 [95% CI: 0.85–0.95], and an optimal sensitivity and specificity of 84.91% and 94.32%, respectively, in the training cohort. In the independent validation cohort, the AUC was 0.88 [95% CI: 0.83–0.92], along with an optimal sensitivity of 81.5% and specificity of 92.9%. The model sensitivity for stage I–II ESCC was 78.4%, which was slightly lower than the sensitivity of the model (85.7%) for stage III–IV ESCC.Conclusion:The dual-target panel based on cfDNA showed excellent performance for detecting ESCC and might be an alternative strategy for screening ESCC.

29 Jan 2024·International Journal of Systematic and Evolutionary Microbiology

Parachitinimonas caeni gen. nov., sp. nov., a novel member of family Burkholderiaceae isolated from activated sludge collected in Shenzhen, PR China

Article

Author: Chen, Han ; Dai, Jingcheng ; Li, Shuyang ; Wang, Xin ; Qiu, Dongru ; Wang, Jing ; Wang, Shanhui ; Jiao, Dian ; Liu, Yaqi ; Li, Yan ; Yuan, Siliang

A Gram-stain-negative, strictly aerobic and filamentous bacterial strain, designated as DQS-5T, was isolated from the activated sludge of a municipal sewage treatment plant in Shenzhen, PR China. Optimal growth was observed at 28 °C and pH 7.5. Catalase and oxidase activities were detected. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DQS-5T was most closely related to the genera

Chitinimonas

and

Chitinivorax

(91.0–93.4 % and 92.5 % 16S rRNA gene sequence similarity, respectively) and was close to the member of the family

Burkholderiaceae

. The complete genome sequence of strain DQS-5T contains 5 653 844 bp and 57.3 mol% G+C. The average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity values between the genome of strain DQS-5T and those of its close relatives were 75.9–77.2, 19.0–20.3 and 57.2–61.8 %, respectively. Chemotaxonomic analysis of strain DQS-5T indicated that the sole respiratory quinone was ubiquinone-8, the predominant cellular fatty acids were C16 : 0 and summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c), and the major polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, aminophospholipid and aminolipid. The phylogenetic, genotypic, phenotypic and chemotaxonomic data demonstrate that strain DQS-5T represents a novel species in a novel genus within the family

Burkholderiaceae

, for which the name Parachitinimonas caeni gen. nov., sp. nov., is proposed. Strain DQS-5T (=KCTC 92788T=CCTCC AB 2022320T) is the type and only strain of P. caeni.

100 Deals associated with Wuhan Ammunition Life-tech Co., Ltd.

100 Translational Medicine associated with Wuhan Ammunition Life-tech Co., Ltd.

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