Sichuan Kelun Pharmacy Institute Co., Ltd.

OBJECTIVE To establish a RP-HPLC method for the determination of sodium lactate in compound sodium lactate and glucose injection and provide reference for revision of method recorded in Chinese Pharmacopoeia.METHODS The separation was performed on a Shim-pack VP-ODS C₁₈ anal. column (4.6 mm × 250 mm, 5 μm), and the mobile phase was formic acid-dicyclohexylamine-water (1:1:998, V/V) at a flow rate of 1.0 mL·min^-1.The detection wavelength was set at 210 nm, and the column temperature was maintained at 40°C.The loading volume was 20 μL.The RP-HPLC method and the cation exchange resin method adopted by Chinese Pharmacopoeia were both used to determine the content of sodium lactate in compound sodium lactate and glucose injection, and the results were compared.RESULTS The linear range of the RP-HPLC method was 0.51-6.07 mg·mL^-1 (r = 0.9999).The average recovery was 99.47% (RSD = 0.75%, n = 9).CONCLUSION The established method of RP-HPLC can be used for the determination of sodium lactate in compound sodium lactate and glucose injection.