Delving into the Latest Updates on DIREVO Industrial Biotechnology GmbH with Synapse

Overview

Continuous fermentation was applied to the production of recombinant human chymotrypsinogen B (hCTRB) by the methylotrophic yeast Pichia pastoris as a tool for the kinetic anal. of growth and product formation.Using methanol as the sole source of carbon, energy, and induction, cell growth could be described by a non-competitive MONOD approach.Maximum growth rate μ_max was determined to 0.084 h^-1 and the K_M-value for methanol to 0.22 g·L^-1, resp.With respect to product formation, a similar model was established exhibiting a methanol concentration of 0.13 g·L^-1 as the K_M-value and a maximum biomass-specific product-formation rate of π_max = 0.23 mg·g^-1·h^-1.The production of hCTRB was strictly growth-coupled.The data provided covers the range of methanol concentrations between 0 and 4 g·L^-1.Substrate concentrations exceeding this upper value led to a complete collapse of product formation.This change in phenotype turned out to be irreversible indicating a genetic instability of transformed Pichia pastoris caused by excess methanol.

Speciality Chemicals Magazine

Mannanase engineering for fiber degradation

Author: Kensch, Oliver

The multi-parameter optimization of a mannanase by high throughput automated confocal fluorimetric screening at Direvo Industrial Biotechnol. is addressed.Trichoderma endo-β-mannanase was engineered using Direvo's proprietary DNA shuffling methods alongside various random library generation and screening approaches to optimize mannanase.A seven-fold increase in product yield at 70° was reached when the product yields of the wild-type and the variant 16.0 were compared.The 16.0 variant is suitable to successfully release mannose from palm kernel meal and as it is improved in thermostability as well, it can be employed in many (galacto)mannan hydrolysis uses at raised temperatures

100 Deals associated with DIREVO Industrial Biotechnology GmbH

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100 Translational Medicine associated with DIREVO Industrial Biotechnology GmbH

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Pipeline

Pipeline Snapshot as of 16 May 2025

The statistics for drugs in the Pipeline is the current organization and its subsidiaries are counted as organizations,Early Phase 1 is incorporated into Phase 1, Phase 1/2 is incorporated into phase 2, and phase 2/3 is incorporated into phase 3

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