Objective: To study the therapeutic effects of chloramphenicol prednisone liniment on acute atopic dermatitis in mice. Methods: Forty-eight mice were equally divided into the blank group, model group, control group and exptl. groups (low dose group, medium dose group and high dose group), with 8 cases in each group. Except for the blank group, atopic dermatitis model was established by dinitrochlorophenone solution in all the other 5 groups. The blank group and model group were given normal saline. The control group was given 1% pimecrolimus cream. The exptl. groups (low dose group, medium dose group and high dose group) were given corresponding concentration of chlorine resp., once a day, 7 d for 1 course of treatment. After treatment of 24 h, the therapeutic effects of chloramphenicol prednisone liniment on atopic dermatitis in mice were evaluated by indicators of serum IFN-γ, IL-4, IL-5, IgE, ear swelling, thymus indicator, spleen indicator and skin thickness. Results: After treatment of 7 d, the levels of IL-4, IL-5 and IgE in the exptl. group were significantly lower than those in the model group (P<0.05), the levels of IFN-γ were significantly higher than those in the model group (P<0.05), the degree of ear swelling, thymus indicator, spleen indicator and skin thickness in the medium dose group and high dose group were significantly lower than those in the model group (P<0.05). Conclusion: Chloramphenicol prednisone liniment has anti-bacterial, anti-inflammatory, anti-allergic and immune-regulating effects, which can improve the symptoms of atopic dermatitis, regulate the balance of Th1/Th2 cells, and has significant therapeutic effects on atopic dermatitis in mice. Chloramphenicol prednisone liniment can be used for the treatment of similar inflammatory skin diseases.