Last update 01 Nov 2024

RUNX2 x Alkaline phosphatase

Last update 01 Nov 2024

Basic Info

Related Targets

RUNX2Alkaline phosphatase

Drugs associated with RUNX2 x Alkaline phosphatase

Oxy-133

Target

Alkaline phosphatase x RUNX2

Mechanism

Alkaline phosphatase stimulants [+1]

Active Org.

MAX BioPharma, Inc.

Originator Org.

MAX BioPharma, Inc.

Active Indication

Bone Diseases

Inactive Indication-

Drug Highest PhasePreclinical

First Approval Ctry. / Loc.-

First Approval Date20 Jan 1800

100 Clinical Results associated with RUNX2 x Alkaline phosphatase

100 Translational Medicine associated with RUNX2 x Alkaline phosphatase

0 Patents (Medical) associated with RUNX2 x Alkaline phosphatase

1,602

Literatures (Medical) associated with RUNX2 x Alkaline phosphatase

01 Jan 2025·Bioactive Materials

Ultrasound-generated bubbles enhance osteogenic differentiation of mesenchymal stromal cells in composite collagen hydrogels

Article

Author: Franceschi, Renny T. ; Abeid, Bachir A. ; Zhang, Man ; Macpherson, Aidan ; Maji, Somnath ; Emara, Ziyad ; Kazyak, Karsyn ; Chiravuri, Anjali ; Abeid, Bachir A ; Midekssa, Firaol S ; Quesada, Carole ; Aliabouzar, Mitra ; Franceschi, Renny T ; Midekssa, Firaol S. ; Kent, Robert N. ; Baker, Brendon M. ; Kent, Robert N ; Fabiilli, Mario L ; Pinch, Abigail ; Baker, Brendon M ; Fabiilli, Mario L.

Hydrogels can improve the delivery of mesenchymal stromal cells (MSCs) by providing crucial biophysical cues that mimic the extracellular matrix. The differentiation of MSCs is dependent on biophysical cues like stiffness and viscoelasticity, yet conventional hydrogels cannot be dynamically altered after fabrication and implantation to actively direct differentiation. We developed a composite hydrogel, consisting of type I collagen and phase-shift emulsion, where osteogenic differentiation of MSCs can be non-invasively modulated using ultrasound. When exposed to ultrasound, the emulsion within the hydrogel was non-thermally vaporized into bubbles, which locally compacted and stiffened the collagen matrix surrounding each bubble. Bubble growth and matrix compaction were correlated, with collagen regions proximal (i.e., ≤ ∼60 μm) to the bubble displaying a 2.5-fold increase in Young's modulus compared to distal regions (i.e., > ∼60 μm). The viability and proliferation of MSCs, which were encapsulated within the composite hydrogel, were not impacted by bubble formation. In vitro and in vivo studies revealed encapsulated MSCs exhibited significantly elevated levels of RUNX2 and osteocalcin, markers of osteogenic differentiation, in collagen regions proximal to the bubble compared to distal regions. Additionally, alkaline phosphatase activity and calcium deposition were enhanced adjacent to the bubble. An opposite trend was observed for CD90, a marker of MSC stemness. Following subcutaneous implantation, bubbles persisted in the hydrogels for two weeks, which led to localized collagen alignment and increases in nuclear asymmetry. These results are a significant step toward controlling the 3D differentiation of MSCs in a non-invasive and on-demand manner.

01 Jan 2025·Archives of Oral Biology

Effects of icariin on dental pulp stem cells and its potential applications in dentin repair

Article

Author: Kim, Sunil ; Lee, Jong-Min ; Jung, Han-Sung ; Lee, Sukjoon ; Elhakim, Ahmed ; Kim, Ukseong ; Kim, Euiseong

OBJECTIVESAs dental pulp therapy evolves towards regenerative approaches, biomolecules such as icariin, derived from Epimedium flowers, are being evaluated for their therapeutic potential. This study investigates icariin's effectiveness in promoting odontogenic differentiation in human dental pulp stem cells (hDPSCs) in vitro and as a pulp-capping agent in vivo.DESIGNThe study explored the effects of icariin on hDPSCs at concentrations of 10, 20, and 40 µM. Cell viability and migration assays were conducted to evaluate cytotoxicity and chemotaxis. Odontogenic differentiation was assessed using alkaline phosphatase staining and alizarin red S (ARS) staining, complemented by real-time PCR and Western blot analyses of key markers such as RUNX family transcription factor 2 (RUNX2), collagen type I alpha 1 chain (COL1A1), alkaline phosphatase (ALPL), and dentin sialophosphoprotein (DSPP). Additionally, the in vivo effects of icariin were tested in a rat maxillary molar model, where icariin-treated collagen sponges were used for direct pulp capping to evaluate its potential to induce reparative dentin formation.RESULTSIcariin showed no cytotoxic effects on hDPSCs at any tested concentration, enhanced migratory activity in a dose-dependent manner, and significantly increased alkaline phosphatase activity and calcium deposition. Gene and protein expression analyses revealed a dose-dependent increase in odontogenic differentiation markers in icariin-treated hDPSCs. In vivo, icariin effectively promoted reparative dentin formation in exposed rat pulp.CONCLUSIONSIcariin enhances odontogenic differentiation of hDPSCs and has promising potential as a pulp-capping agent for vital pulp therapy.

01 Nov 2024·International Journal of Biological Macromolecules

The release behavior and in vitro osteogenesis of quercetin-loaded bioactive glass/hyaluronic acid/sodium alginate nanocomposite paste

Article

Author: Sohrabi, Mehri ; Hesaraki, Saeed ; Shahrezaee, Mostafa ; Shams-Khorasani, Alireza

Injectable pastes based on bioactive compounds and natural polymers are of interest in non-invasive bone surgeries. Several quantities of quercetin (100, 150, and 200 μM) were added to a sol-gel derived mesoporous bioactive glass. Injectable pastes based on quercetin-loaded bioactive glass, sodium alginate, and hyaluronic acid were prepared. Aggregated nanoparticles of bioactive glass and quercetin-loaded bioactive glass with mesoporous morphologies were confirmed by TEM and BET techniques. The quercetin release study was assessed in phosphate-buffered solution medium over 200 h and the obtained data were fitted by different eqs. A sustained release of quercetin was found, in which a better regression coefficient was achieved using Weibull equation. Human-derived mesenchymal stem cells were utilized to determine alkaline phosphatase activity and bone-related protein expression by western blotting and real-time PCR evaluations. Quercetin-loaded pastes increased the levels of alkaline phosphatase activity and the expression of Collagen-1, Osteopontin, Osteocalcin, and Runx2 proteins in a concentration-dependent manner. Due to the mesoporous architecture and high specific surface area of bioactive glass, the paste made of these particles and sodium alginate/hyaluronic acid macromolecules is appropriate matrix for quercetin release, resulting in promoted osteogenesis. The further in vivo studies can support the osteogenesis capacity of the quercetin-loaded paste.

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