MT2 x MT1L

Last update 08 May 2025

Basic Info

Related Targets

MT2MT1L

Drugs associated with MT2 x MT1L

CA3220842

Patent Mining

Target

MT1L x MT2

Mechanism-

Active Org.

Unigen Co., Ltd.

Originator Org.

Unigen Co., Ltd.

Active Indication

Cardiovascular Diseases [+4]

Inactive Indication-

Drug Highest PhaseDiscovery

First Approval Ctry. / Loc.-

First Approval Date20 Jan 1800

100 Clinical Results associated with MT2 x MT1L

100 Translational Medicine associated with MT2 x MT1L

0 Patents (Medical) associated with MT2 x MT1L

Literatures (Medical) associated with MT2 x MT1L

28 Feb 2021·British Journal of Nutrition

Sensitivity and reliability of zinc transporter and metallothionein gene expression in peripheral blood mononuclear cells as indicators of zinc status: responses toex vivozinc exposure and habitual zinc intake in humans

Article

Author: McClung, Holly L ; Armstrong, Nicholes J ; McClung, James P ; Hennigar, Stephen R ; Berryman, Claire E ; Anderson, Bradley J ; Kelley, Alyssa M ; Karl, J Philip

AbstractZn is an essential nutrient for humans; however, a sensitive biomarker to assess Zn status has not been identified. The objective of this study was to determine the reliability and sensitivity of Zn transporter and metallothionein (MT) genes in peripheral blood mononuclear cells (PBMCs) to Zn exposureex vivoand to habitual Zn intake in human subjects. In study 1, human PBMCs were cultured for 24 h with 0–50 µmZnSO4with or without 5 µmN,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN), and mRNA expression ofSLC30A1-10,SLC39A1-14,MT1subtypes (A,B,E,F,G,H,L,MandX),MT2A,MT3andMT4mRNA was determined. In study 2, fifty-four healthy male and female volunteers (31·9 (sd13·8) years, BMI 25·7 (sd2·9) kg/m2) completed a FFQ, blood was collected, PBMCs were isolated and mRNA expression of selected Zn transporters and MT isoforms was determined. Study 1:MT1E,MT1F,MT1G,MT1H,MT1L,MT1M,MT1X,MT2AandSLC30A1increased with increasing concentrations of Zn and declined with the addition of TPEN. Study 2: Average daily Zn intake was 16·0 (sd5·3) mg/d (range: 9–31 mg/d), and plasma Zn concentrations were 15·5 (SD2·8) μmol/l (range 11–23 μmol/l). PBMCMT2Awas positively correlated with dietary Zn intake (r0·306,P= 0·03) and total Zn intake (r0·382,P< 0·01), whereas plasma Zn was not (P> 0·05 for both). Findings suggest thatMT2AmRNA in PBMCs reflects dietary Zn intake in healthy adults and may be a component in determining Zn status.

01 Dec 2020·Molecular ImmunologyQ3 · MEDICINE

Corneal tissue induces transcription of metallothioneins in monocyte-derived human macrophages

Q3 · MEDICINE

Article

Author: Lapp, Thabo ; Lange, Clemens ; Schlunck, Günther ; Fan, Jiaqi ; Hildebrand, Antonia ; Boneva, Stefaniya ; Böhringer, Daniel ; Kammrath Betancor, Paola ; Wolf, Julian ; Zhuang, Xinyu ; Maier, Philip ; Schwämmle, Melanie ; Reinhard, Thomas

PURPOSEImmune reactions following corneal transplantation are the most common cause of transplant failure. However, the underlying mechanisms of corneal graft rejection are not yet fully understood but increasing evidence points to a crucial role of the innate immune system in this context. Using a human in vitro model, we aimed to assess the response of human macrophages to stimulation with human corneal tissue and whether corneal endothelial cells (CEC) have immune-modulating properties.METHODSHuman monocytes were isolated from peripheral blood mononuclear cells and differentiated into monocyte-derived macrophages (MDM). A standardized protocol was used for disaggregation of human corneas into fragments of defined sizes. MDMs were stimulated using processed corneal material with or without CEC. Lipopolysaccharide (LPS) or interferon-gamma (IFNγ) served as controls. RNA sequencing was applied to analyze the impact of differential stimulation of MDMs on their transcriptional profile. RNA sequencing results were validated using digital PCR.RESULTSThe transcriptional profile of MDMs was significantly modulated by the type of stimulus used for MDM activation as well as by the individual MDM donor. LPS- or IFNγ-stimulation resulted in distinct transcriptional alterations compared to unstimulated MDMs including an upregulation of various cytokines such as CCL3, 4, 5, 19 or CXCL9. Corneal tissue induced the differential expression of 45 genes when compared to unstimulated MDMs, with several metallothioneins (MTs) among the upregulated factors (MT1A, MT1E, MT1F, MT1G, MT1H, MT1L, MT1M, MT1X, MT2A). This effect was independent of the presence or absence of CEC. PCR validation confirmed induction of 3 different metallothioneins (MT1G, MT1H and MT2A) in MDMs stimulated by corneal tissue.CONCLUSIONSThe MDM in vitro model proved to be a robust tool to study the effects of LPS, IFNγ and corneal tissue homogenates on the transcriptional activity of MDM. Human macrophages showed a distinct upregulation of various MTs when challenged with human corneal allogen with or without corneal endothelium, which might have an immune-modulatory effect. As a general observation, it appears that in MDM-based studies a significant donor-dependent effect on the transcriptional profile of MDMs needs to be considered and adjusted before downstream analysis.

01 Jan 2012·NeuroimmunomodulationQ4 · MEDICINE

Melatonin Membrane Receptor Type MT1 Modulates Cell-Mediated Immunity in the Seasonally Breeding Tropical Rodent Funambulus pennanti

Q4 · MEDICINE

Article

Author: Ahmad, Raise ; Haldar, Chandana ; Gupta, Sameer

Objective: Despite the evidence for melatonin membrane receptors (MT1R and MT2R) on lymphoid tissues in a wide range of seasonal breeders, their specific potency has never been compared and correlated with cell-mediated immunity. Methods: We used luzindole, a nonselective MT2R antagonist, and 4-phenyl-2-propionamidotetralin (4P-PDOT), a selective MT2R antagonist, to assess the potency of the melatonin receptors MT1 and MT2 in melatonin-induced immunity under both in vivo as well as in vitro conditions. Results: Physiological doses (25 µg/100 g body weight in vivo and 100 and 500 pg/ml in vitro) of melatonin upregulated both MT1R and MT2R expression as well as splenocyte proliferation, while higher doses (100 and 500 µg/100 g body weight in vivo and 1 ng/ml in vitro) downregulated splenocyte proliferation and the expression of both receptors. Luzindole antagonized the expression of both MT1R and MT2R in a dose-dependent manner under in vivo as well as in vitro conditions, while 4P-PDOT blocked the expression of MT2R only during both experimental conditions. Splenocyte proliferation and IL-2 secretion (in vitro) followed the MT1R expression pattern, while the MT2R expression pattern showed no definite relation with either splenocyte proliferation or IL-2 secretion under in vivo and in vitro conditions. Conclusion: Immune function in tropical rodents is directly regulated by melatonin via its high-affinity membrane receptor MT1. MT1R plays a directive role in mediating splenocyte proliferation and IL-2 release, while the MT2R subtype appears not to be required for the immunoenhancing role of melatonin.

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