Last update 01 Nov 2024

UNG

Last update 01 Nov 2024

Basic Info

Synonyms

DGU, HIGM4, UDG

+ [6]

Introduction

Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.

Drugs associated with UNG

Target UDG (XPose Therapeutics)

Target

UNG

Mechanism

UNG modulators

Active Org.

Xpose Therapeutics, Inc.Startup

Originator Org.

Xpose Therapeutics, Inc.Startup

Active Indication

Neoplasms

Inactive Indication-

Drug Highest PhasePreclinical

First Approval Ctry. / Loc.-

First Approval Date20 Jan 1800

100 Clinical Results associated with UNG

100 Translational Medicine associated with UNG

0 Patents (Medical) associated with UNG

1,598

Literatures (Medical) associated with UNG

01 Jan 2025·Analytical Biochemistry

Uracil base PCR implemented for reliable DNA walking

Article

Author: Chen, Hong ; Gu, Yinwei ; Li, Haixing ; Tian, Bingkun ; Wang, Rongrong

PCR-based DNA walking is of efficacy for capturing unknown flanking genomic sequences. Here, an uracil base PCR (UB-PCR) with satisfying specificity has been devised for DNA walking. Primary UB-PCR replaces thymine base with uracil base, resulting in a primary PCR product composed of U-DNAs. A single-primer (primary nested sequence-specific primer) single-cycle amplification, using the four normal bases (adenine, thymine, cytosine, and guanine) as substrate, is then performed on the primary PCR product. Clearly, only those U-DNAs, ended by the primary nested sequence-specific primer at least at one side, will produce the corresponding normal single strands. Next, the single-cycle product undergoes uracil-DNA glycosylase treatment to destroy the U-DNAs, while the normal single strands are unaffected. Afterward, secondary even tertiary PCR is performed to exclusively enrich the target product. The feasibility of UB-PCR has been checked by obtaining unknown sequences bordering the three selected genetic sites.

22 Oct 2024·Analytical Chemistry

Sequentially Activated-Dumbbell DNA Nanodevices for Accurate Detection of Uracil-DNA Glycosylase via PER-Based Orthogonal Signal Outputs

Article

Author: Zhou, Xue-Mei ; Zhuo, Ying ; Yuan, Ruo ; Yang, Xia ; Chai, Ya-Qin ; Li, Ying-Huan ; Yuan, Xiao-Qing ; Lei, Yan-Mei

Accurate and reliable detection of uracil-DNA glycosylase (UDG) activity is crucial for clinical diagnosis and prognosis assessment. However, current techniques for accurately monitoring UDG activity still face significant challenges due to the single input or output signal modes. Here, we develop a sequentially activated-dumbbell DNA nanodevice (SEAD) that enables precise and reliable evaluation of UDG activity through primer exchange reactions (PER)-based orthogonal signal output. The SEAD incorporates a double-hairpin structure with a stem containing two deoxyuridine (dU) sites for target recognition and two preblocked primer binding regions for target amplification and signal output. Upon UDG recognition of dU, the SEAD can be cleaved by apurinic/apyrimidinic endonuclease 1 (APE1), generating two different hairpins with exposed primer binding regions. These hairpins serve as templates to initiate the parallel PER, enabling the extending of two different amplification products: a long single-stranded DNA (ssDNA) with repetitive sequences and a short ferrocene-labeled ssDNA with complementary sequences. These products further self-assemble into DNA nano-strings in an orthogonal manner that act as an electrochemiluminescence signal switch, enabling precise detection of low-abundance UDG. This work develops a sequential input and orthogonal output strategy for accurately monitoring UDG activity, highlighting the significant potential in cancer diagnosis and treatment.

16 Oct 2024·Chemistry – An Asian Journal

Enzyme‐Powered, Label‐Free DNA Walker for Uracil‐DNA Glycosylase Detection at Single‐Cell Level

Article

Author: Zong, Haotian ; Wang, Shuaijing ; Li, Jiayue ; Wang, Zhenguang ; Zhou, Yi ; Li, Wei

AbstractUracil‐DNA glycosylase (UDG) plays a crucial role in the removal of damaged uracil bases, thereby upholding genetic stability and integrity. An enzyme‐powered, label‐free DNA walker was devised for UDG activity detection. Initially, a label‐free DNA track, incorporating a gold nanoparticle (AuNP), multiple hairpin structures, and various swing arms, was engineered for walking mechanism. The hairpin structure was meticulously crafted to include a G‐quadruplex sequence, enabling the generation of a label‐free fluorescence signal. The swing arm remained inert in the absence of UDG, but became activated upon the introduction of UDG, thereby initiating the enzyme‐powered walking process and generating significant dissociative G‐quadruplex sequences. By integrating a selective fluorescent dye into the design, an enhanced label‐free fluorescence response was achieved. The proposed DNA walker presented a direct and label‐free approach for UDG detection, demonstrating exceptional sensitivity with a detection limit of 0.00004 U/mL. Using the uracil glycosylase inhibitor (UGI) as an inhibitory model, inhibitor assay was conducted with satisfactory precision. Furthermore, successful analysis of cellular UDG at the single‐cell level was accomplished. Consequently, the developed DNA walker serves as a label‐free, selective, and sensitive tool for UDG activity assessment, showing great potential for applications in disease diagnosis, inhibitor screening, and biomedical investigations.

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