Last update 19 Sep 2024

UNG

Last update 19 Sep 2024

Basic Info

Synonyms

DGU, HIGM4, UDG

+ [6]

Introduction

Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.

Drugs associated with UNG

Target UDG (XPose Therapeutics)

Target

UNG

Mechanism

UNG modulators

Active Org.

Xpose Therapeutics, Inc.Startup

Originator Org.

Xpose Therapeutics, Inc.Startup

Active Indication

Neoplasms

Inactive Indication-

Drug Highest PhasePreclinical

First Approval Ctry. / Loc.-

First Approval Date20 Jan 1800

100 Clinical Results associated with UNG

100 Translational Medicine associated with UNG

0 Patents (Medical) associated with UNG

1,591

Literatures (Medical) associated with UNG

01 Oct 2024·Pathology - Research and Practice

Comprehensive exploration on the role of base excision repair genes in modulating immune infiltration in low-grade glioma

Article

Author: Kumar, Santosh ; Kumari, Subhadra ; Muthuswamy, Srinivasan ; P, Parthipan

INTRODUCTIONGlioma is a brain tumour occurring in all age groups but common in adults. Despite advances in the understanding of tumours, we cannot improve the survival of the patients and do not have an appropriate biomarker for progression and prognosis prediction. The base excision repair mechanism maintains the integrity of the genome, preventing tumour formation. However, continuous chemical damage to the cells results in mutations that escape the repair mechanism and support tumour growth. The tumour microenvironment in cancer is crucial in determining the tumour growth, development, and response to treatments. The present study explored the significance of Base Excision Repair genes (BER) in modulating the tumour microenvironment.METHODSWe used the publically available data sets from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) to explore the role of the base excision repair gene in the modulating tumour microenvironment. The data was analysed for the expression of base excision repair genes, their correlation with the immune markers, their prognostic potential, and enrichment analysis to understand the pathways they modulate in low-grade glioma (LGG) progression.RESULTSThe analysis showed BER genes contribute an integral role in the overall and disease-free survival of LGG. Genes like MUTYH, PNKP, UNG and XRCC1 showed a correlation with the immune infiltration levels and a significant correlation with various immune markers associated with different immune cells, including tumour-associated macrophages. MUTYH, UNG and XRCC1 correlated with IDH1 mutation status, and functional enrichment analysis showed that these genes are enriched in several pathways like Wnt, PD-1 and Integrin signalling.CONCLUSIONOur findings suggest that the BER genes MUTYH, PNKP, UNG and XRCC1 can potentially be prognostic biomarkers and highly correlate with the immune cells of the tumour microenvironment.

21 Aug 2024·ACS Measurement Science Au

Detection of Uracil-Excising DNA Glycosylases in Cancer Cell Samples Using a Three-Dimensional DNAzyme Walker

Author: Zhang, Hongquan ; Weinfeld, Michael ; Le, X. Chris ; Tao, Jeffrey

DNA glycosylase dysregulation is implicated in carcinogenesis and therapeutic resistance of cancers.Thus, various DNA-based detection platforms have been developed by leveraging the base excision activity of DNA glycosylases.However, the efficacy of DNA-based methods is hampered due to nonspecific degradation by nucleases commonly present in cancer cells and during preparations of cell lysate.In this report, we describe a fluorescence-based assay using a specific and nuclease-resistant three-dimensional DNAzyme walker to investigate the activity of DNA glycosylases from cancer cell lysates.We focus on DNA glycosylases that excise uracil from deoxyuridine (dU) lesions, namely uracil DNA glycosylase (UDG) and single-stranded monofunctional uracil DNA glycosylase (SMUG1).Limits of detection for detecting UDG and SMUG1 in buffer were 3.2 pM and 3.0 pM, resp.The DNAzyme walker detected uracil-excision activity in diluted cancer cell lysate from as low as 48 A549 cells.of the UDG inhibitor experiments demonstrate that UDG is the predominant uracil-excising glycosylase in A549 cells.Approx. 500 μM of UDG is present in each A549 cell on averageNo fluorescence was generated in the samples lacking DNAzyme activation, indicating there was no nonspecific nuclease interference.The ability for the DNAzyme walker to respond to glycosylase activity illustrates the potential use for DNAzyme walker technol. to monitor and study biochem. processes involving glycosylases.

01 Aug 2024·Gene

Regulatory role of PDK1 via integrated gene analysis of mitochondria-immune response in periodontitis

Article

Author: Yang, Zhan ; Chen, Shuhong ; Wu, Jiayu ; Sun, Xiaoyu ; Liang, Guang ; Liu, Jiefan ; Cao, Gang ; Wu, Tong ; Wu, Shengzhuang ; Zhao, Zheyu ; Mao, Yixin ; Huang, Shengbin ; Hu, Chaoming ; Guo, Chen ; Xu, Xiangwei

AIMSTo investigate the association between mitochondrial events and immune response in periodontitis and related regulatory genes.MAIN METHODSGene expression profiles in gingival tissues were retrieved from the Gene Expression Omnibus. Mitochondria-immune response-related differentially expressed genes (MIR-DEGs) between the healthy and periodontitis samples were determined. WGCNA, GO, and KEGG were used to investigate the function and the enriched pathways of MIR-DEGs. The correlation between MIR-DEGs expression and clinical probing pocket depth was analyzed. The MIR-DEGs were further identified and verified in animal samples. A periodontitis model was established in C57BL/6 mice with silk ligation. Micro-computed tomography was used to assess alveolar bone loss. Western blot, quantitative real-time polymerase chain reaction, and immunohistochemical analyses further validated the differential expression of the MIR-DEGs.KEY FINDINGSA total of ten MIR-DEGs (CYP24A1, PRDX4, GLDC, PDK1, BCL2A1, CBR3, ARMCX3, BNIP3, IFI27, and UNG) were identified, the expression of which could effectively distinguish patients with periodontitis from the healthy controls. Enhanced immune response was detected in the periodontitis group with that in the healthy controls, especially in B cells. PDK1 was a critical MIR-DEG correlated with B cell immune response and clinical periodontal probing pocket depth. Both animal and clinical periodontal samples presented higher gene and protein expression of PDK1 than the control samples. Additionally, PDK1 colocalized with B cells in both animal and clinical periodontal tissues.SIGNIFICANCEMitochondria participate in the regulation of the immune response in periodontitis. PDK1 may be the key mitochondria-related gene regulating B-cell immune response in periodontitis.

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UNG

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