Last update 01 Nov 2024

NS1 x env

Last update 01 Nov 2024

Basic Info

Related Targets

NS1env

Drugs associated with NS1 x env

ZIKV-NS1-LAV

Target

NS1 x env

Mechanism

NS1 inhibitors [+1]

Active Org.-

Originator Org.

Moderna, Inc. [+3]

Active Indication-

Inactive Indication

Zika Virus Infection

Drug Highest PhasePending

First Approval Ctry. / Loc.-

First Approval Date20 Jan 1800

100 Clinical Results associated with NS1 x env

100 Translational Medicine associated with NS1 x env

0 Patents (Medical) associated with NS1 x env

Literatures (Medical) associated with NS1 x env

25 Mar 2024·Biological Chemistry

The Zika virus infection remodels the expression of the synaptotagmin-9 secretory protein

Article

Author: Rosano, Germán ; Luppo, Victoria ; Cantoia, Alejo ; Morales, María Alejandra ; Gardiol, Daniela ; Leiva, Santiago ; Bugnon Valdano, Marina ; Fabbri, Cintia

AbstractThe exact mechanisms involved in flaviviruses virions’ release and the specific secretion of viral proteins, such as the Non Structural protein-1 (NS1), are still unclear. While these processes might involve vesicular transport to the cell membrane, NS1 from some flaviviruses was shown to participate in viral assembly and release. Here, we assessed the effect of the Zika virus (ZIKV) NS1 expression on the cellular proteome to identify trafficking-related targets that may be altered in the presence of the viral protein. We detected an increase in the synaptotagmin-9 (SYT9) secretory protein, which participates in the intracellular transport of protein-laden vesicles. We confirmed the effect of NS1 on SYT9 levels by transfection models while also detecting a significant subcellular redistribution of SYT9. We found that ZIKV prM-Env proteins, required for the viral particle release, also increased SYT9 levels and changed its localization. Finally, we demonstrated that ZIKV cellular infection raises SYT9 levels and promotes changes in its subcellular localization, together with a co-distribution with both Env and NS1. Altogether, the data suggest SYT9’s implication in the vesicular transport of viral proteins or virions during ZIKV infection, showing for the first time the association of synaptotagmins with the flavivirus’ life cycle.

01 Jun 2017·Journal of Virological MethodsQ4 · MEDICINE

Enhancing the sensitivity of Dengue virus serotype detection by RT-PCR among infected children in India

Q4 · MEDICINE

Article

Author: Anmol Chandele ; Syed Fazil Ahamed ; Murali Krishna Kaja ; Rosario Vivek ; Anita Shet ; Kaustuv Nayak ; Shalini Kotabagi

Dengue surveillance relies on reverse transcription-polymerase chain reaction (RT-PCR), for confirmation of dengue virus (DENV) serotypes. We compared efficacies of published and modified primer sets targeting envelope (Env) and capsid-premembrane (C-prM) genes for detection of circulating DENV serotypes in southern India. Acute samples from children with clinically-diagnosed dengue were used for RT-PCR testing. All samples were also subjected to dengue serology (NS1 antigen and anti-dengue-IgM/IgG rapid immunochromatographic assay). Nested RT-PCR was performed on viral RNA using three methods targeting 654bp C-prM, 511bp C-prM and 641bp Env regions, respectively. RT-PCR-positive samples were validated by population sequencing. Among 171 children with suspected dengue, 121 were dengue serology-positive and 50 were dengue serology-negative. Among 121 serology-positives, RT-PCR detected 91 (75.2%) by CprM654, 72 (59.5%) by CprM511, and 74 (61.1%) by Env641. Among 50 serology-negatives, 10 (20.0%) were detected by CprM654, 12 (24.0%) by CprM511, and 11 (22.0%) by Env641. Overall detection rate using three methods sequentially was 82.6% (100/121) among serology-positive and 40.0% (20/50) among serology-negative samples; 6.6% (8/120) had co-infection with multiple DENV serotypes. We conclude that detection of acute dengue was enhanced by a modified RT-PCR method targeting the 654bp C-prM region, and further improved by using all three methods sequentially.

01 Dec 2015·Virologica Sinica

Molecular characterization of DENV-3 circulating during the post-monsoon period of 2013–14 in Delhi, India

Letter

Author: Kumari, Roop ; Venkatesh, Srinivas ; Bhattacharya, Dipesh ; Rai, Arvind ; Sharma, Pankaj ; Singh, Priyanka ; Chhabra, Mala ; Mittal, Veena

The present study characterizes the recently circulating DENV-3 strain in the metropolitan city of Delhi during post monsoon period of 2013-14. Partial molecular characterization of 12 DENV3 isolates was carried out on the basis of envelope (E) and non-structural 1 (NS1) gene regions. Phylogenetic analysis showed all these 12 isolates grouped under lineage III of genotype III with recent isolates from China and Pakistan. The point mutation L430I in the Env region appears to be the unique molecular signature for the 2013 strains. Another unique substitution, I167V in NS1 protein was observed in a single isolate among those 12 samples. The study describing the molecular characterization could be significant if these unique substitutions cause the poor B cell responses.

Analysis

Perform a panoramic analysis of this field.

view full example data

Chat with Hiro

Get started for free today!

Accelerate Strategic R&D decision making with Synapse, PatSnap’s AI-powered Connected Innovation Intelligence Platform Built for Life Sciences Professionals.

Start your data trial now!

Synapse data is also accessible to external entities via APIs or data packages. Empower better decisions with the latest in pharmaceutical intelligence.

Bio

Bio Sequences Search & Analysis

Chemical

Chemical Structures Search & Analysis