Last update 01 Nov 2024

EGF x aFGF

Last update 01 Nov 2024

Basic Info

Related Targets

EGFaFGF

Drugs associated with EGF x aFGF

RG-8803

Target

EGF x aFGF

Mechanism

EGF inhibitors [+2]

Active Org.-

Originator Org.

Repligen Corp.

Active Indication-

Inactive Indication

Diabetic Retinopathy [+1]

Drug Highest PhaseDiscontinued

First Approval Ctry. / Loc.-

First Approval Date20 Jan 1800

100 Clinical Results associated with EGF x aFGF

100 Translational Medicine associated with EGF x aFGF

0 Patents (Medical) associated with EGF x aFGF

300

Literatures (Medical) associated with EGF x aFGF

01 Mar 2024·Genes to Cells

The optimized priming effect of FGF‐1 and FGF‐2 enhances preadipocyte lineage commitment in human adipose‐derived mesenchymal stem cells

Article

Author: Tarapongpun, Tanakorn ; Onlamoon, Nattawat ; Tabu, Kouichi ; Chuthapisith, Suebwong ; Taga, Tetsuya

AbstractThe cell‐assisted lipotransfer technique, integrating adipose‐derived mesenchymal stem cells (ADMSCs), has transformed lipofilling, enhancing fat graft viability. However, the multipotent nature of ADMSCs poses challenges. To improve safety and graft vitality and to reduce unwanted lineage differentiation, this study refines the methodology by priming ADMSCs into preadipocytes—unipotent, self‐renewing cells. We explored the impact of fibroblast growth factor‐1 (FGF‐1), fibroblast growth factor‐2 (FGF‐2), and epidermal growth factor (EGF), either alone or in combination, on primary human ADMSCs during the proliferative phase. FGF‐2 emerged as a robust stimulator of cell proliferation, preserving stemness markers, especially when combined with EGF. Conversely, FGF‐1, while not significantly affecting cell growth, influenced cell morphology, transitioning cells to a rounded shape with reduced CD34 expression. Furthermore, co‐priming with FGF‐1 and FGF‐2 enhanced adipogenic potential, limiting osteogenic and chondrogenic tendencies, and possibly promoting preadipocyte commitment. These preadipocytes exhibited unique features: rounded morphology, reduced CD34, decreased preadipocyte factor 1 (Pref‐1), and elevated C/EBPα and PPARγ, alongside sustained stemness markers (CD73, CD90, CD105). Mechanistically, FGF‐1 and FGF‐2 activated key adipogenic transcription factors—C/EBPα and PPARγ—while inhibiting GATA3 and Notch3, which are adipogenesis inhibitors. These findings hold the potential to advance innovative strategies for ADMSC‐mediated lipofilling procedures.

05 Oct 2023·European journal of medicinal chemistry

Discovery of novel thymidylate synthase (TS) inhibitors that influence cancer angiogenesis and metabolic reprogramming in NSCLC cells.

Article

Author: Dong, Gang ; Lin, Qi-Qi ; Meng, Fan-Hao ; Xue, Wen-Han ; Deng, Mei-Yan ; Li, Xin-Yang ; Li, Yu-Heng ; Guo, Jing-Si

Based on previous work, further search for more effective and less damaging thymidylate synthase (TS) inhibitors was the focus of this study. After further optimization of the structure, in this study, a series of (E)-N-(2-benzyl hydrazine-1-carbonyl) phenyl-2,4-deoxy-1,2,3,4-tetrahydro pyrimidine-5-sulfonamide derivatives were synthesized and reported for the first time. All target compounds were screened by enzyme activity assay and cell viability inhibition assay. On the one hand, the hit compound DG1 could bind directly to TS proteins intracellularly and promote apoptosis in A549 and H1975 cells. Simultaneously, DG1 could inhibit cancer tissue proliferation more effectively than Pemetrexed (PTX) in the A549 xenograft mouse model. On the other hand, the inhibitory effect of DG1 on NSCLC angiogenesis was verified both in vivo and in vitro. In parallel, DG1 was further uncovered to inhibit the expression of CD26, ET-1, FGF-1, and EGF by angiogenic factor antibody microarray. Moreover, RNA-seq and PCR-array assays revealed that DG1 could inhibit NSCLC proliferation by affecting metabolic reprogramming. Collectively, these data demonstrated that DG1as a TS inhibitor could be promising in treating NSCLC angiogenesis, deserving further investigation.

01 Aug 2023·Journal of Biomedical Materials Research Part A

Positive effect of acellular amniotic membrane dressing with immobilized growth factors in skin wound healing

Article

Author: Habibi‐Anbouhi, Mahdi ; Kazemi‐Lomedasht, Fatemeh ; Laleh, Mahsa ; Tahernejad, Mahrokh ; Asefnejad, Azadeh ; Bonakdar, Shahin ; Golkar, Majid

AbstractThe human amniotic membrane dressing has been shown to accelerate the wound healing process in the clinic. In this study, heparin was conjugated to a human Acellular Amniotic Membrane (hAAM) to provide affinity binding sites for immobilizing growth factors. To study the acceleration of the wound healing process, we bound epidermal growth factor and fibroblast growth factor 1 to heparinized hAAMs (GF‐Hep‐hAAMs). The heparinized hAAMs (Hep‐hAAMs) were characterized by toluidine blue staining and infrared spectroscopy. The quality control of hAAM was performed by hematoxylin staining, swelling capacity test and biomechanical evaluation. The cytotoxicity, adhesion, and migration in vitro assays of GF‐Hep‐hAAMs on L‐929 fibroblast cells were also studied by MTT assay, scanning electron microscopy, and scratch assay, respectively. Finally, in vivo skin wound healing study was performed to investigate the wound closure rate, re‐epithelization, collagen deposition, and formation of new blood vessels. The results showed that GF‐Hep‐hAAMs enhance the rate of wound closure and epidermal regeneration in BALB/c mice. In conclusion, GF‐Hep‐hAAMs could accelerate the wound healing process, significantly in the first week.

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