Arctigenin

The storage and processing of Litopenaeus vannamei are often challenged by the freeze-thaw (F-T) cycle phenomenon. This study delved into the influence of pretreatment with l-arginine (Arg) and l-lysine (Lys) on the myofibrillar proteins oxidation and quality of shrimp subjected to F-T cycles. Arg and Lys pretreatment notably improved water-holding capacity (WHC), textural integrity as well as the myofibrillar structure of the shrimps. A lesser reduction in the amounts of immobile and bound water was found in the amino acid-treated groups, and the oxidation of lipids and proteins were both decelerated. Molecular simulation results indicated that Arg and Lys could form hydrogen and salt-bridge bonds with myosin, enhancing the stability of Litopenaeus vannamei. The study concludes that Arg and Lys are effective in alleviating the adverse effects of F-T cycles on the quality of Litopenaeus vannamei, and provides a new solution for the quality maintenance during storage and processing.

A quantitative analysis method was established using high-performance liquid chromatography (HPLC) to simultaneously determine five lignans in the total lignan extract (TLE) from Trachelospermi Caulis at 280 nm, including nortracheloside, tracheloside, nortrachelogenin, trachelogenin, and arctigenin. The method demonstrated good linearity for each lignan within their respective ranges, with precision, stability, and repeatability meeting the criteria for quantitative analysis. Tracheloside, the most abundant compound in the TLE, effectively inhibited the release of inflammatory factors IL-6 and IL-17, and suppressed the migration MH7A cells in vitro. Furthermore, tracheloside reduced the production of key inflammatory factors, including COX-2, IL-6, IL-17, MMP2, MMP3, MMP9, JNK, p-JNK, p38, and p-p38 in TNF-α induced MH7A cells, thereby inhibiting the IL-17/MAPK signaling pathway, and thus contributing to its anti-rheumatoid arthritis effects. This study represents the first report on the simultaneous quantification of five major lignans from Trachelospermi Caulis and the anti-inflammatory properties of tracheloside.