Isoxsuprine Hydrochloride

Pharmaceutical pollution in wastewater poses significant environmental and public health concerns worldwide. Chloramphenicol (CP), an antibiotic widely used in medical and veterinary applications, is among the active pharmaceutical ingredients (APIs) frequently detected in aquatic environments. This study explored the encapsulation of chloramphenicol API in contaminated wastewater using rationally designed fibrations based on the silver metal ion-directed self-assembly of fibrillator-type self-assembling ligand (ANS-3). We further investigated the removal of various commonly prescribed drugs, including antibiotics such as β-lactam (amoxicillin), fluoroquinolone (ciprofloxacin), aminoglycoside (neomycin), and tetracycline; antiparasitic agents with antiprotozoal properties (praziquantel and metronidazole); nonsteroidal anti-inflammatory drugs (NSAIDs) such as phenylbutazone and ketoprofen; the vasodilator isoxsuprine; amphiphilic antidepressants (amitriptyline); and the antiviral drug amantadine. The findings validated the crucial influence of polar multifunctionality and structural complexity in enhancing interactions with Ag.ANS-3 matrix, emphasizing its potential for efficient drug sequestration. First, picolinic acid (PA) and phenylalanine (F) were evaluated for their ability to form fibrillar structures, and their morphological characterization revealed well-defined fibrillar networks with varying degrees of porosity and interconnectivity. Then, the strategic inclusion of leucine in synthesizing ANS-3 facilitated the formation of robust fibrillar networks, employing its hydrophobic interactions to drive the self-assembly process. Finally, the encapsulation of APIs was evaluated using Ag(I) metal ion-driven ANS-3 based self-assembled nanofibrous material. This research contributes to the development of innovative physicochemical wastewater treatment strategies for environmental remediation and validates the importance of rational design in encapsulation-based wastewater remediation technologies.

Dried urine spots have recently been proposed as an alternative matrix in the anti-doping field. Drying urine may open the opportunity to limit microbial and thermal degradation of the prohibited substances during transportation to the anti-doping laboratories without the need for refrigeration or freezing. In this study, a multi-targeted initial testing procedure was developed for the determination of 237 prohibited drugs/metabolites from 11 different classes in dried urine spots. The comparability between two different microsampling techniques (i.e., Whatman^® FTA DMPK-C cards and Mitra^® tips) was evaluated. The developed method was then used to evaluate the stability of the target compounds in urine for 7 days under different environmental conditions to simulate the transportation of the urine samples from the collection sites to anti-doping laboratories. Sample preparation consists of (i) extraction of the analytes from the collection device using a mixture of acetonitrile/methanol (1/1) for 30 min at 40 °C, (ii) enzymatic hydrolysis, and (iii) sample concentration by solid-phase extraction. Analysis was performed using liquid chromatography coupled to high-resolution mass spectrometry. The entire workflow was validated in terms of specificity (analytes were distinguishable from the matrix interferences), sensitivity (only with the Mitra^® tips the limits of detection comply with the World Anti-Doping Agency's requirements for the majority of the target compounds), carry-over (no signals in the negative urine injected after the positive urine), matrix effect (16-28% for Mitra^® tips and 22-35% for DMPK-C cards), and extraction yield (Mitra^® tips: 51-88%; DMPK-C cards: 40-76%). As proof of concept, authentic urine samples were analyzed: results obtained in dried urine were compared with those of fluid urine, providing good agreement. Stability studies showed that the target compounds were stable for the whole duration of the study (7 days) at -20 and 4 °C in both fluid and dried urine. At 50 °C or at 20-25 °C, several thiazide-based compounds were completely degraded to their degradation product in the first 24 h or after 3-4 days in fluid urine, whereas in dried urine the compounds were detectable for the entire duration of the study.