Delving into the Latest Updates on Universidade Federal do Rio Grande with Synapse

Overview

Tags

Infectious Diseases

Small molecule drug

Disease domain score

A glimpse into the focused therapeutic areas

Technology Platform

Most used technologies in drug development

Targets

Most frequently developed targets

Disease Domain	Count

Infectious Diseases	1

Top 5 Drug Type	Count

Small molecule drug	1

Top 5 Target	Count

Bacterial efflux pump	1

BACKGROUNDThe development of therapies and vaccines for various diseases often necessitates the analysis of cellular immunity. However, unlike other rodents, the limited availability of reagents for Syrian hamsters restricts immunological analysis, particularly in the determination of serum effector molecules such as cytokines. In this study, we aim to produce and characterize the cytokines IFN-γ, TGF-β, IL-6, IL-10, and TNF-α from Syrian hamsters in recombinant form and to generate polyclonal antibodies against them in rats.METHODS AND RESULTSCytokine transcript sequences were cloned into expression vectors in E. coli. Recombinant proteins were produced, purified through affinity chromatography, and characterized by Western blot using an anti-6xHis monoclonal antibody. Rats were immunized with the recombinant proteins to generate polyclonal antibodies (pAbs). These pAbs were characterized by Western blot and titrated by indirect ELISA. The recombinant cytokines rTNF-α, rIL-10, rIFN-γ, rTGF-β, and rIL-6 were produced and specifically recognized at their expected molecular weights of 22.3 kDa, 19.8 kDa, 18.9 kDa, 11.8 kDa, and 22.9 kDa. pAbs were produced and demonstrated the ability to specifically recognize their target proteins with titers of 409,600 (rIL-10), 204,800 (rTNF-α), 102,400 (rIL-10), 51,200 (rTGF-β), and 25,600 (rIFN-ɣ).CONCLUSIONSThe reagents produced represent a starting point for developing immunoassays to detect hamster cytokines, facilitating the analysis of cellular immunity in this biomodel.

01 Dec 2024·Aquaculture International

Assessment of mycotoxins found in farmed fish feed

Author: Tesser, Marcelo Borges ; Nogueira, Wesclen Vilar ; Buffon, Jaqueline Garda

Abstract: Filamentous fungi, mainly those belonging to genera Aspergillus, Fusarium, and Penicillium, the main producers of mycotoxins and responsible for food and feed contamination, exhibit metabolites that are highly toxic to animals and humans.This scoping review compiles data on occurrence of mycotoxins in fish feed, assesses distribution of metabolites across continents by the hierarchical cluster anal., and establishes risks posed to the main global orders of farmed fish by exposure to mycotoxins.Resulting data showed that fish of different orders in both fingerling and juvenile phases are exposed to high concentrations of mycotoxins.

01 Dec 2024·Environmental Pollution

Imposex incidence in gastropod species from the Colombian Caribbean Coast reveals continued and widespread tributyltin contamination after its global ban

Article

Author: Johnson Restrepo, Boris ; Fernández Maestre, Roberto ; Fillmann, Gilberto ; Braga Castro, Ítalo ; Meza-Morelos, Dairo

Organotin compounds (OTCs), such as tributyltin (TBT) and triphenyltin (TPhT), are released in aquatic environments from antifouling coatings and can cause imposex, an abnormal condition where female snails develop male sexual characteristics. This study investigates temporal variations in imposex incidence along the Colombian Caribbean coast following the 2008 global ban on TBT-based antifouling paints. Over a 12-year period, we assessed imposex in 1,384 adults snails from six species (58% female and 42% male) during 2012, 2016, and 2023. In 2012, just four years after the ban, imposex incidence in some sites reached 100% in the neogastropods Purpura patula, Stramonita haemastoma, Stramonita rustica, and Thais deltoideia. In 2016, imposex in some sites reached 81% for the mesogastropod Strombus pugilis and 39% for the neogastropod Melongena melongena. By 2023, imposex decreased for the first four neogastropods, persisted for S. pugilis, and increased for M. melongena only in Cartagena Bay and the Tolú marina. These findings indicate a general decline in imposex over time, aligning with the ban, but also suggest potential new sources of contamination as imposex rates increased in some previously unaffected areas.

100 Deals associated with Universidade Federal do Rio Grande

Login to view more data

100 Translational Medicine associated with Universidade Federal do Rio Grande

Login to view more data

Corporation Tree

Boost your research with our corporation tree data.

login

or

view full example data

Boost your research with our corporation tree data.

Pipeline

Pipeline Snapshot as of 25 Nov 2024

The statistics for drugs in the Pipeline is the current organization and its subsidiaries are counted as organizations,Early Phase 1 is incorporated into Phase 1, Phase 1/2 is incorporated into phase 2, and phase 2/3 is incorporated into phase 3

Preclinical

1

Login to view more data