To establish an new assay method for rutin and Salvianolic acid B in Xueshuan Xinmaining tablets by HPLC.The Agilent HC C18 column (4.6 mm × 150 mm, 5 μm) was adopted with a mobile phase of Methanol-acetonitrile-0.5% formic acid(30:9:61), the flow rate was 0.7 mL/min-1, the wavelength of detection was set at 257 nm.Salvianolic acid B's linearity was obtained within the range of 0.207-2.07 μg (r = 0.9996), and the average recovery(n = 6) was 98.6%, and RSD was 1.0%.Rutin's linearity was obtained within the range of 0.2028 ∼ 2.028 μg (r = 0.9999), and the average recovery (n = 6) was 99.5%, and RSD was 0.5%.The method is simple, reliable and reproducible, and can be used to control the quality of Xueshuan Xinmaining tablets.