Delving into the Latest Updates on Wuhan Medical College with Synapse

Overview

OBJECTIVETo study the expression of TNF-alpha in the cholesteatoma and elucidate the role of TNF-alpha in the destruction of bone.METHODAll samples (5 microns paraffin sections) from 25 cholesteatoma cases and 10 normal cases were examined by immunohistochemical SABC method and analysed by computer image.RESULTIn 25 cholesteatoma cases, TNF-alpha expressed in the cytoplasm of the full epithelial tissue cells, subcutaneous inflammatory cells and fibroblast. In contrast, the normal cases, there were 6 cases week positive, 4 cases negative. The results of the computer image quantitative analysis system showed that the mean optical density of TNF-alpha was 0.1326 +/- 0.0022 in the cholesteatoma epithelial tissue and 0.0868 +/- 0.0014 in normal skin epithelial tissue respectively, both of which had significantly differentiation (P < 0.05).CONCLUSIONTNF-alpha may be a factor of the destruction of bone of cholesteatoma.

Journal of clinical otorhinolaryngology

[The study of TNF-alpha destructive action to the bone in human middle cholesteatoma].

Author: Xue Q ; Song H ; Jin K

OBJECTIVE To study the expression of TNF-alpha in the cholesteatoma and elucidate the role of TNF-alpha in the destruction of bone. METHOD All samples (5 microns paraffin sections) from 25 cholesteatoma cases and 10 normal cases were examined by immunohistochemical SABC method and analysed by computer image. RESULT In 25 cholesteatoma cases, TNF-alpha expressed in the cytoplasm of the full epithelial tissue cells, subcutaneous inflammatory cells and fibroblast. In contrast, the normal cases, there were 6 cases week positive, 4 cases negative. The results of the computer image quantitative analysis system showed that the mean optical density of TNF-alpha was 0.1326 +/- 0.0022 in the cholesteatoma epithelial tissue and 0.0868 +/- 0.0014 in normal skin epithelial tissue respectively, both of which had significantly differentiation (P < 0.05). CONCLUSION TNF-alpha may be a factor of the destruction of bone of cholesteatoma.

Zhongguo Gonggong Weisheng

Study on genotype of hantavirus strains isolated from different areas in Hubei province

Author: Qu, Hong ; Yang, Jijiang ; Liu, Hanyan ; Zhao, Min ; Liu, Dongying ; Ding, Xiaohua ; Yang, Zhanqiu ; Hou, Wei

The M genomic segment of 30 strains of Hantavirus (HV) isolated from different areas in Hubei province was analyzed to understand the genetic characteristics and the genomic function of hantavirus (HV) in Hubei province.According to Genebank software of HV, the type-specific primers were designed corresponding to the conserved sequences in the G1 region of M genomic segment of HV.Heminested RT-PCR and restriction mapping methods were applied to type and digest 30 strains of HV isolated from different areas in Hubei province.Thirty strains isolated from different areas in Hubei province were typed as 21 HTNV and 9 SEOV, resp.The restriction mappings of 21 isolates digested by Hinf I and HaeIII were consistent with those of 76/118 standard strain, but it was different from those of H-114 strain, of which digested by both HaeIII in only one segment in Wuhan and Honghu City and Hinf I in three segments in Yingcheng, Jianxia, Huangpi, and Macheng city, resp.Then all HTNV were digested by Hinc II except the above strain in Yingoheng city.In addition, 9 isolates were digested by Hinf I in two segments or EcoRI in two segments while not being digested by Hae III, which were not consistent with those of R₂₂ strain.Hubei province was an mixed epidemic areas of HFRS; the M genomic segment of SEOV was stable, but that of HTNV no stability; and distinct hantaviruses could coexist in either different or the same geog. or ecol. sores in Hubei province.

100 Deals associated with Wuhan Medical College

Login to view more data

100 Translational Medicine associated with Wuhan Medical College

Login to view more data

Corporation Tree

Boost your research with our corporation tree data.

login

or

view full example data

Boost your research with our corporation tree data.

Pipeline

Pipeline Snapshot as of 07 Mar 2025

The statistics for drugs in the Pipeline is the current organization and its subsidiaries are counted as organizations,Early Phase 1 is incorporated into Phase 1, Phase 1/2 is incorporated into phase 2, and phase 2/3 is incorporated into phase 3

Other

2

Login to view more data