The intermediate of remdesivir was synthesized from 2-Et butanol and L-alanine by esterification, amination and hydroxylation reaction, the reaction conditions, such as catalyst type, catalyst amount, acid binding agent type, reaction solvent and reaction temperature, were examined to give the preferable process, the total yield was 40%, purity was 99.6%.The structure was characterized by ∼1H-NMR and MS.This method has the advantages of easy to obtain raw materials, mild reaction conditions, simple operation, low cost and environmental friendliness, and is suitable for industrial production