Objective: To establish the method for simultaneous determination of oleanolic acid and ursolic acid in traditional Chinese medicine of Herba Rabdosiae Serrae. Methods: The chromatog. column was CAPCELL PAK C18 MG II (with octadecylsilane chem. bonded silica as the packed column), and it was adopted with the mobile phase of acetonitrile-methanol-0.6% ammonium acetate (67:12:21). The detection wavelength was set at 210 nm with the flow rate of 1 mL·min-1, the column temperature of 30°C and the injection volume of 20 μL. Results: Oleanolic acid and ursolic acid exhibited satisfied linear relationship in the range of 5.066∼50.664 μg·mL-1 and 9.735∼97.352 μg·mL-1, resp. (r=0.998). Meanwhile, the RSD of precision, stability and repeatability were less than 2.0%. The average recovery rates were 99.78% (RSD=0.56%) and 100.68% (RSD=0.67%), resp. Conclusion: The proposed method of HPLC was simple, reproducible and accurate, and can be applied for quality control of medicinal material of Herba Rabdosiae Serrae.