TRBC2 x TRBC1 x CD3

Last update 23 Jan 2025

Basic Info

Related Targets

TRBC2TRBC1CD3

Drugs associated with TRBC2 x TRBC1 x CD3

BTCE-TRBC2(Meiji Seika Pharma)

Target

CD3 x TRBC1 x TRBC2

Mechanism

CD3 stimulants [+2]

Active Org.

Meiji Seika Pharma Co., Ltd.

Originator Org.

Meiji Seika Pharma Co., Ltd.

Active Indication

T-Cell Leukemia [+1]

Inactive Indication-

Drug Highest PhasePreclinical

First Approval Ctry. / Loc.-

First Approval Date20 Jan 1800

100 Clinical Results associated with TRBC2 x TRBC1 x CD3

100 Translational Medicine associated with TRBC2 x TRBC1 x CD3

0 Patents (Medical) associated with TRBC2 x TRBC1 x CD3

Literatures (Medical) associated with TRBC2 x TRBC1 x CD3

04 Jul 2022·Laboratory MedicineQ4 · MEDICINE

Reliable Detection of T-Cell Clonality by Flow Cytometry in Mature T-Cell Neoplasms Using TRBC1: Implementation as a Reflex Test and Comparison with PCR-Based Clonality Testing

Q4 · MEDICINE

Article

Author: Waldron, Deirdre ; O’Brien, David ; Quinn, Fiona ; Vandenberghe, Elizabeth ; Smyth, Laura

AbstractObjectiveThe T-cell receptor β constant region 1 (TRBC1) antibody can identify T-cell clonality and distinguish pathological from normal T cells. This study aims to establish optimal cutpoints for establishing monotypia and validate the diagnostic abilities of the TRBC1 antibody when used as a reflex test in conjunction with an existing T-cell antibody panel.Materials and MethodsWe used 46 normal peripheral blood specimens and examined 8 patients with reactive lymphoproliferations to determine the normal biological range of TRBC1 on CD4+ and CD8+ T cells. We also evaluated 43 patient specimens that were submitted for investigation of a lymphoproliferative disorder for CD2/CD3/CD4/CD5/CD7/CD8/CD16/CD26/CD45/CD56/TCR αβ/TCR γδ, along with TRBC1 expression. The results were compared to TCR gene rearrangement patterns using polymerase chain reaction (PCR) analysis.ResultsStatistical analysis established differing cutoff points for establishing monotypia dependent on restricted TRBC1 or TRBC2 usage. Direct comparison with molecular analysis indicated that no specimen identified with the restricted expression of TRBC1 was reported as polyclonal by PCR with a concordance rate of 97% between a clonal PCR result and monotypic TRBC1 expression.ConclusionIncorporation of the TRBC1 antibody using statistically derived cutoff points in a reflex setting for the evaluation of a suspected T-cell neoplasm improves the identification of clonal T-cell populations by flow cytometry and correlates well with molecular methods.

01 May 2021·Cytometry Part B: Clinical CytometryQ4 · MEDICINE

Flow cytometric evaluation of TRBC1 expression in tissue specimens and body fluids is a novel and specific method for assessment of T‐cell clonality and diagnosis of T‐cell neoplasms

Q4 · MEDICINE

Article

Author: Berg, Holly ; Jevremovic, Dragan ; Olteanu, Horatiu ; Corley, Heidi ; Otteson, Gregory E. ; Horna, Pedro ; Shi, Min

AbstractBackgroundFlow cytometric detection of T‐cell clonality is challenging. The current available methodology for T‐cell receptor (TCR) Vβ repertoire evaluation is a complex assay and has limited sensitivity especially for detecting low levels of disease. Therefore, there is an unmet need for a reliable, simple, and rapid assay to identify T‐cell clonality. The rearrangement of the TCRB gene involves the random and mutually exclusive expression of one of two constant β chain genes (TRBC1 and TRBC2), analogous to the kappa and lambda gene utilization by B cells.MethodsHere, we used a single TRBC1 antibody, in conjunction with other T‐cell associated markers, to detect T‐cell clonality in tissue biopsies and body fluids. A total of 143 tissue/body fluid specimens from 46 patients with a definitive diagnosis of a T‐cell neoplasm and 97 patients with no T‐cell malignancy were analyzed with a cocktail of monoclonal antibodies including CD2/CD3/CD4/CD5/CD7/CD8/CD45/TCRγδ/TRBC1.ResultsWe examined TRBC1 expression on neoplastic T‐cell populations identified based on their immunophenotypic aberrancies, and monotypic TRBC1 expression was identified in all 46 known T‐cell lymphoma cases. We applied a similar gating strategy to the 97 cases without T‐cell neoplasms, and arbitrarily dissected T‐cell populations into immunophenotypically distinct subsets; in this group, we found that all cases revealed an expected polytypic TRBC1 expression in all subsets.ConclusionsSingle TRBC1 antibody detection of T‐cell clonality by flow cytometry is a simple, rapid, and robust assay that could be routinely utilized in flow cytometric laboratories.

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