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Assessing the quality of surface waters is essential for identifying changes in freshwater ecosystems and supporting the planning/proposing of measures to mitigate polluting sources. However, many studies involving the identification of pathogenic bacteria and/or their resistance profile to antimicrobial agents need a more holistic approach to conditioning or modulating factors. Thus, we apply different multivariate statistical techniques to the data set from the Capibaribe River's surface water, one of the most important in the Northeast of Brazil. Our data, taken together, suggest that the waters of the Capibaribe River have been suffering impacts associated with different human activities. Due to its flow crossing a large urban area, different sources are contributing to the contamination/pollution of its aquatic ecosystem, whose multivariate analysis allowed us to identify site-dependent characteristics that reflect the degree and type of human influence. The study of physical-chemical and chemical parameters reveals the influence of the high load of effluents (industrial and domestic) on the chemical and microbiological quality of the waters sampled at the SS4 site. On the other hand, the antimicrobial resistance profile of the isolates evaluated, especially at SS1, SS2, and SS3 sites, provides a comprehensive sample of the "resistome" present in the fecal content of thousands of people living in the region surrounding the Capibaribe River. The presence of enterobacteria in water indicates contamination of fecal origin. It represents a public health problem since the waters of the Capibaribe River can be a source of dissemination and persistence of bacteria resistant to humans and the environment. In conclusion, our study provides a more comprehensive understanding of the relationships between surface water, basic sanitation, antibiotic exposure, bacterial gene transfer, and human colonization, whether in the context of the region studied or other locations.

Curcumin loaded β-CD inclusion complexes (CCDs) embedded in intrinsically active rhamnosomes (CCDRs) were developed as alternative antimicrobials at the nano-scale to control ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.) pathogens.The physicochem. anal. of CCDRs demonstrated that the addition of glycolipids (rhamnolipids) during the development of liposomes (phospholipids based) provided stability while the average size (158±5 nm) and polydispersity index (PDI) values of 0.2 demonstrated homogenous population of rhamnosomes.The incorporation of rhamnolipids with phospholipids increased the zeta potential to -45±1 mV with 76±1 % encapsulation efficiency for curcumin with enhanced photochem. and storage stability.In vitro, release studies demonstrated sustained release of curcumin due to its incorporation in β-CD mol. buckets, which were loaded in the aqueous liposomal core.These CCDRs with intrinsic antibacterial and antibiofilm potential enhanced the antimicrobial spectrum against ESKAPE pathogens demonstrating synergism between curcumin and rhamnolipids, by targeting multiple sites of bacterial cells and biofilms as compared to conventional antibiotics.Our study outlines that rhamnolipids and curcumin loaded inclusion complexes together offer an exciting potential for functionalized liposomal delivery systems that would be promising for the development of effective alternative therapeutics against ESKAPE pathogens.