The Summit HPLC from Dionex in combination with Chromeleon software and the autosampler ASI 100(T) offers the possibility of carrying out a (DIN-compliant) quantification by automatic calibration using variable volumes of a standard The automatic injection of these volumes omits weighing and pipetting work and makes it possible to monitor measuring uncertainty by means of special system suitability tests in routine operations. In the investigated range, this method gave homogeneous variances. Unfortunately, this advantage of the autosampler is not yet fully supported by the Chromeleon software because the the calibration function cannot evaluate with prognosis intervals. Future versions of the software should support this.