In this work, a simple and versatile chemosensor receptor TZPYZ was synthesized through the combination of thiazole with pyrazine-2-carbohydrazide, resulting in a confirmed chem. structure via various anal. techniques including FT-IR, 1H, and 13C NMR Spectroscopy, as well as High-Resolution Mass Spectroscopy anal.TZPYZ exhibits specific colorimetric and photoluminescent responses to Ag+ ions in a solvent solution consisting of DMSO and H2O (7:3, volume/volume).Upon addition of Ag+ ions, noticeable changes in absorption spectra occur, resulting in a visible color change from pale yellow to blue.Addnl., an enhanced emission intensity with wavelength at 523 nm, when excited at 410 nm.Notably, TZPYZ demonstrated exceptional selectivity for Ag+ ions over other metal cations, achieving a detection limit (LOD) of 10.6 x 10-9 M and 6.74 x 10-9 M and using the UV-visible & photoluminescent titration method.Interference studies indicated minimal disruption from other metal ions on emission at 523 nm, highlighting TZPYZ discerning capability for Ag+ ions.With a binding affinity of 3.622 x 10-11 M-1, TZPYZ proved effective in detecting Ag+ ions across various water samples, showcasing its practical utility.The mechanism of interaction between TZPYZ and Ag+ ions was investigated using various exptl. techniques, including Job′s plot, Benesi-Hildebrand investigations, 1H NMR, and HRMS anal.Test strips coated with TZPYZ showed selective detection of Ag+ ions, indicating its potential for on-site applications.Furthermore, DFT computations provided insights into the structural and electronic properties of TZPYZ and its complex with Ag+ ions, further elucidating the binding mechanism and stability of the complex.In addition, TZPYZ demonstrated compatibility with biol. systems, as fluorescence imaging tests on MCF-7 breast cancer cells confirmed both its non-cytotoxic nature and its proficiency in detecting intracellular silver ions.Based on these findings, TZPYZ is highlighted as a highly sensitive and selective chemosensor for Ag+ ions, with promising applications in environmental anal. and bioimaging.