Mayinglong Pharmaceutical Group Co., Ltd.

The preparation technol. of safflower injections was studied. With hydroxysafflor yellow A, kaempferol and adenosine as the standards, the contents of the components in safflower injections with different preparation process were determined by HPLC, and the contents were also determined with freeze-drying process instead of the high temperature treatment. The contents of hydroxysafflor yellow A and kaempferol were decreased in the process of water extraction and alc. deposition of water deposition, and those were significantly decreased by high temperature process compared with those by freeze-drying, while the high temperature and freeze-drying processes showed no influence on the content of adenosine. The preparation technol. of safflower injections is unreasonable, and the active ingredients with heat sensitivity can be preserved by freeze-drying.

The purification process parameters of total flavonoids from Pericarpium Citri Reticulatae Viride with macroporous resin were investigated. With the content of total flavonoids as the evaluation index, the optimum purification process parameters were screened by the exptl. study of dynamic adsorption and desorption. The conditions of adsorption process were as follows: the herbal concentration was 0.25 g·ml^-1, the sample quantity was 1.1 g·g^-1 resin, and the ratio of diameter to height was 1:8. The desorption process was as follows: 10 BV and 50% alc. eluted the macro porous resin column after removing the impurities with 5 BV water. The content of total flavonoids in the extracts was above 90%. Macroporous resin shows application value in the purification of total flavonoids from Pericarpium Citri Reticulatae Viride.

An HPLC method for the determination of paeoniflorin in Chimu effervescent tablets was established. The determination was performed on a Zobax Eclipse XDB-C₁₈ (250 mm×4.6 mm, 5 μm) column, acetonitrile-water (11:89) was the mobile phase with the flow rate of 1.0 mL·min^-1 and the column temperature of 30°C, and the detection wavelength was at 230 nm. The linear range of paeoniflorin was within the range of 5.06-80.96 μg·mL^-1 (r=0.9999). The average recovery was 98.30% (RSD=0.91%, n=6). The method was accurate, simple and rapid, and could be used for the determination of paeoniflorin in Chimu effervescent tablets.