Various environmental factors such as pH, temperature and initial glucose concentration were investigated for enhancing cell growth in fermentations of Phellinus linteus WI-001, a producer of polysaccharides with potent anticancer activities. Optimal pH and temperature were around 5.5 and 28°C, resp. Relatively little variation of pH was observed ranging between 5.5 and 6.5 during the whole fermentation period. Maximum cell concentration and specific growth rate were investigated in the media containing initial glucose concentrations of 0.5%, 1%, 2%, 3% and 4%. High initial glucose concentration enhanced biomass production but showed neg. effect on specific growth rate. In bioreactor experiments with various feeding strategies, increases of 28% and 42% in final cell concentration were obtained as compared to conventional batch process, by adopting pulse and continuous supplement of 2% glucose solution, resp.